Thursday, 31 July 2025

Pilot Study on the Usefulness of Crude Extract from Spirometra erinacei in Serodiagnosis of Human Sparganosis | Chapter 10 | Microbiology and Biotechnology Research: An Overview Vol. 4

 

Sparganosis is a zoonotic parasitic disease caused by the larvae (spargana) of the genus Spirometra, which is worldwide distributed. More than 60 species of Spirometra have been identified, and over 2000 cases have been reported. A seroepidemiologic examination for the detection of anti-Spirometra erinacei in serum was carried out in normal inhabitants in Babati district, Tanzania. Sera were tested by enzyme-linked immunosorbent assay (ELISA) for the antibodies.  Antigen of adult Spirometra was extracted by using the Bradford assay method (1976). The extract was used as an antigen, and specific antibody levels in sparganosis sera reacted positively. Sera of 31 inhabitants from Babati were tested by enzyme-linked immunosorbent assay (ELISA). Out of 31 participants' sera, 19 (61.3%) reacted positively to crude extracts of adult Spirometra. This result showed that crude extracts of adult S. erinacei could be used as a diagnostic antigen of human sparganosis.

Author(s) Details

 

Nicholas J. Kavana
Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences, P.O. Box 175, Ifakara, Tanzania.

 

Please see the book here:- https://doi.org/10.9734/bpi/mbrao/v4/3577

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