Background and Objectives: Chick embryos are commonly used in
research to investigate the negative impacts of various drugs. The drug
lixisenatide is superior to other GLP1 receptor agonists for treating type 2
diabetes mellitus. It is recommended to take 20 µg of lixisenatide daily for
type 2 diabetes mellitus. However, there are no clear human dose
recommendations based on age and weight. It is important to conduct further
research on the teratogenic effects of lixisenatide to determine the potential
toxicity of the drug for human welfare.
Materials and Methods: It’s an experimental study conducted at the
Department of Anatomy, Santosh Medical College, Ghaziabad, Uttar Pradesh. The
study aimed to investigate the effects of specific parameters in two groups, a
control group, and an experimental group. The expected mean ± SD of the
parameter for the control and experimental groups were found to be 27±5.32 and
32±5.30, respectively, based on a literature survey and pilot study. To achieve
an appropriate sample size, G*Power software was used, with an alpha level of
5% and a power of 95.36%, which resulted in an effect size of 1.961 and a
sample size of 28 for each group experimental and control (A, AC, B, BC, C, CC,
D, DC, E, EC), leading to a total sample size of 280. The chick embryos were
first sacrificed and then dissected, and the brain was separated and kept in a
10% formaldehyde solution. To identify histological characteristics of the
cerebral cortex, tissue was sectioned and stained with H & E stain then
observed by the light and compound microscope were used for sections.
Results: During the experiment, Lixisenatide induced necrotic
changes and cellular condensation in the chick embryo’s cerebral cortex,
specifically in groups C, D, and E. Additionally, there was mild distortion
found in the cortex indicating degenerative changes in experimental group E.
There was a statistically significant increase in histopathological changes
observed in the cerebral cortex as the dose of Lixisenatide increased in each
experimental group (p<0.05).
Conclusion: Insufficient research has been conducted on the
development of side effects of lixisenatide. Despite its widespread use,
observations have revealed that lixisenatide has adverse effects on the
cerebral cortex of chick embryos, including cellular condensation and necrotic
changes, and mild distortion found in the cortex indicating degenerative
changes in experimental groups C, D, and E. Therefore, it is imperative that
the administration of this vital medication is carefully considered to prevent
neuronal complications. Further research is necessary to fully understand the
potential adverse effects of lixisenatide on brain development.
Author(s) Details
Amit
Kumar Srivastava
Department of Anatomy, Santosh Medical College, Ghaziabad, Uttar
Pradesh, India.
Yogesh
Yadav
Department of Anatomy, Saraswati Institute of Medical Sciences,
Hapur, Uttar Pradesh, India.
Swati
Yadav
Department of Anatomy, Santosh Medical College, Gaziabad
Jmtuyeruhj, Uttar Pradesh, India.
Harshita
Bhardwaj
Department of Anatomy, Santosh Medical College, Gaziabad
Jmtuyeruhj, Uttar Pradesh, India.
Ashok
Kumar Gupta
Department of Anatomy, Government Medical College, Saharanpur,
Uttar Pradesh, India.
Please
see the book here:- https://doi.org/10.9734/bpi/ibs/v9/1440
No comments:
Post a Comment