Sunday, 27 July 2025

Mechanism of Oxidative Process Inhibition by Roundup: Role of N-phosphomethylglycine and its Photodecomposition Products | Chapter 6 | Chemistry and Biochemistry: Research Progress Vol. 7

 

The presented material presents the results of studies performed on N-(phosphonomethyl)glycine (NPMG). This compound is the active ingredient of the most widely used herbicide in agricultural practice, Roundup (Glyphosate; Tornado; and many others). The presented study investigates the behaviour and toxicological impact of NPMG, the active ingredient in widely used herbicides such as Roundup, Glyphosate, and Tornado. To assess the hazard of photochemical decomposition products formed under the influence of solar ultraviolet radiation and ozone, NPMG was exposed to hard UV radiation with λ = 250-600 nm with simultaneous bubbling with ozone. After 14.3 hours, NPMG is practically not identified in the solution. The decomposition process is complex and multistage. The toxicological effect of aqueous solutions of NPMG and the products of its photochemical decomposition was studied on test objects: luminous bacteria of the Ecolum series and ciliates Tetrahymena pyriformis. Treatment of NPMG with UV irradiation + ozonation leads to a 10-fold decrease in toxicity. Using lux-biosensors based on Escherichia coli, the ability of NPMG photochemical decomposition products to induce oxidative stress in bacterial cells was studied. A comparative study of genetic effects using two genetic bacterial test systems showed that among the products of NPMG photochemical decomposition, there are substances with weak mutagenic and genotoxic activity. It was shown that NPMG inhibits the activity of horseradish root peroxidase by a competitive type: Ki = 0.1004 mM; Km = 1.776 mM; I50 = 0.302 mM. The obtained data show that NPMG is able to block the antioxidant system and can cause the accumulation of free radicals in warm-blooded organisms, which is an addition to the available literature data on the biological targets of the Roundup herbicide.

 

Author(s) Details

Saratovskikh E. A.
Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Russian Federation.

 

Please see the book here:- https://doi.org/10.9734/bpi/cbrp/v7/5808

No comments:

Post a Comment