A Rapid and Cost-Effective Method for Genomic DNA Extraction from Plant Fungal Pathogens | Chapter 9 | Current Research Progress in Agricultural Sciences Vol. 3

 

This chapter demonstrates a rapid, cost-effective, and safe method for extracting genomic DNA from multiple plant fungal pathogens. Pure cultures of Fusarium equesti, Neoscytalidium dimidiatum, Fusarium proliferatum, and Alternaria alternata, isolated from diseased wheat, grapevine, potato, and lily plants, respectively, were used. The fungal samples were ground with sterilised sand and 2N NaOH, followed by centrifugation to separate sand grains and fungal cellular components from the DNA. The resulting DNA was mixed with Tris buffer (1 M, pH 8). The internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) was successfully amplified, sequenced, and analysed from the extracted DNA of all four pathogens. This safe technique offers a straightforward, efficient, and non-hazardous method to obtain sufficient quantity and quality DNA suitable for molecular assays to identify plant fungi.

 

Author(s) Details

 Adnan A. Lahuf
Department of Plant Protection, College of Agriculture, University of Kerbala, Karbala, Iraq.

 

Ola H. Jaafar
Department of Plant Protection, College of Agriculture, University of Kerbala, Karbala, Iraq.

 

Zainab L. Hameed
Department of Field Crops, College of Agriculture, University of Kerbala, Karbala, Iraq.

 

Please see the book here:- https://doi.org/10.9734/bpi/crpas/v3/2117

Rabbit Angiotensin-converting Enzyme-2: 3D Structural Reconstruction and Comparison with its Human Analog | Chapter 2 | Chemistry and Biochemistry: Research Progress Vol. 5

The transmembrane glycoprotein angiotensin-converting enzyme-2 (ACE2), currently better known as the receptor for the coronavirus S-protein, has a key role in blood pressure regulation in humans and mammals. The polypeptide chain of ACE2 has two segments, which form a large globular hydrophilic domain with carboxypeptidase catalytic activity, and a small hydrophobic transmembrane collectrin-like segment. To explain on a molecular level the association of the S protein to ACE2 when using rabbits as an animal model for studying the coronavirus infection, it is needed to know the 3D structure of the rabbit protein rACE2. The aim of the research is to reconstruct the 3D structure of the catalytic domain of rACE2 using its human analog hACE2 as a model, considering the differences in the primary amino acid sequences of the two enzymes, which have a high identity: 85%. Tertiary structure alignment of rACE2 and hACE2 catalytic domains discloses a coincidence in the α-helix segments, with minor deviations in two unstructured sections of the polypeptide chains. The pH dependences of the electrostatic component of the free energy of the two proteins have similar courses, but the 3D structure of the rabbit analog is somewhat more stable. The significant resemblance in the structure and thermodynamic properties between rACE2 and hACE2 suggests that rabbits can serve effectively as animal models for investigating blood pressure regulation and coronavirus infection, with the findings extrapolable to humans.

 

Author (s) Details

 

Svetlana H. Hristova
Department of Medical Physics and Biophysics, Medical Faculty, Medical University – Sofia, Zdrave Str. 2, Sofia 1431, Bulgaria and Faculty of Physics, Sofia University, 5 James Bourchier Blvd., 1164 Sofia, Bulgaria.

 

Trifon T. Popov
Medical Faculty, Medical University – Sofia, Zdrave Str. 2, Sofia 1431, Bulgaria.

 

Alexandar M. Zhivkov
Scientific Research Center, “St. Kliment Ohridski” Sofia University, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria.

 

 Please see the book here:- https://doi.org/10.9734/bpi/cbrp/v5/4676

Fixed Point Theory for Self and Ciric Type Weak Quasi Contraction Mappings in Different Metric Spaces | Chapter 9 | Research Highlights in Mathematics and Computer Science Vol. 6

 The purpose concerning this communication is to confirm some new unique established point theorems in metric and menger rooms under self and Ciric type -weak quasi shortening mappings. Some important results and drawings to highlight the realized betterings are also supplied.

Author(s) Details:

Rohit Kumar Verma,
Department of Mathematics, Bharti Vishwavidyalaya, Durg, C.G., India.

Please see the link here: https://stm.bookpi.org/RHMCS-V6/article/view/9802