Background: Cervical cancer is common in women in less
developed regions of the world. The plant biomolecules can be employed for
synergistic activity with chemo- and radiotherapy. This combination might
result in reduced toxicity and increased efficacy of the treatment regimen. At
present, more than 8 South African women die from cervical cancer every day.
The anti-cancer activity of the acetone extracts of S. plumosum, T. cilliata
and S. pinnata was assessed using different parameters.
Methods: S. plumosum and S. pinnata were collected from Mankweng and T.
cilliata from Tzaneen in Limpopo province, South Africa. An immortalized human
cell line (HeLa) was used in this study and the cells were cultured and
maintained in RPMI media. Secondary metabolite detection and antioxidant
activity quantification were determined using the DPPH and ferric iron reducing
assays. HeLa cell growth inhibition and mechanistics were assessed by employing
MTT and Annexin-V flous assays.
Results: Observations revealed the presence of phenolic, flavonoids, tannins
steroids and coumarins in all the plants extracts. A high amount of total
phenolic and flavonoid content was detected in S. plumosum and T. cilliata. S.
plumosum extract had the best DPPH scavenging activity and ferric reducing
powers. The Antioxidant activity profile show S. plumosum to contain more
compounds with antioxidant activity as compared to the other two plant
extracts.
Conclusion: Observable concentration dependent cell proliferation inhibition by
test materials was exhibited. The leaf extracts from T. cilliata, S. plumosum
and S. pinnata contain compounds of various polarities with free-radicals,
antioxidant and anti-cancerous activities that may play a beneficial role in
treatment.
Author(s) Details:
Mxolisi Justice Ndlovu,
Department of Biochemistry, Microbiology and Biotechnology Faculty of Sciences and Agriculture, South Africa.
Victor Patrick Bagla,
Department of Biochemistry, Microbiology and Biotechnology Faculty of Sciences and Agriculture, South Africa.
Matlou Phenius Mokgotho,
Department of Biochemistry, Microbiology and Biotechnology Faculty of Sciences and Agriculture, South Africa.
Marema Ephraim Makgatho,
Department of Pathology and Medical Sciences, Faculty of Health Sciences, University of Limpopo, South Africa.
Thabe Moss Matsebatlela,
Department of Biochemistry, Microbiology and Biotechnology Faculty of Sciences and Agriculture, South Africa.
Please see the link here: https://stm.bookpi.org/ANUMS-V5/article/view/13307
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