The present study aimed at carrying out an In silico
analysis of activin gene in guinea fowl and in some avian species, thereby
providing the basis for the use of activin or its downstream target gene for
the improvement of impaired wound healing, and of activin antagonists for the
prevention and treatment of fibrosis and of malignant tumors that over express
activin. Among the avian species, understanding the roles of activin happens to
be a dominant challenge in the genetic evolution due to its complexity in
nature. As a sedentary bird, guinea fowl is more susceptible to local selection
processes and needs a proper genetic study for conservation. The current study
lays the groundwork for the use of activin antagonists to treat and prevent
fibrosis and end malignant tumors that overexpress activin, as well as activin
or its target genes to enhance defective wound healing. The data offered will
function as a foundational instrument for more extensive genetic diversity
investigations aimed at identifying important genes and genetic resources for
poultry breeding programs. This study was done by retrieving hundred (100)
nucleotides and amino acid sequences of activin gene belonging to guinea fowl
and other avians from the GeneBank, aligning the sequences using BLASTp
determined the percent identity and phylogenetic relationship of the activin
gene of guinea fowl and other avians. The shortest ACTIVIN nucleotide sequence
(467bp) was observed in chicken and the longest (39896445) in duck. Using the
comparative sequence analysis, it was observed that ACTIVIN gene of chickens,
turkey and guinea fowl shared percent identity ranging from 91 to 95%. The
percent identity reflects degree of relatedness of species. Although closely
related (90%) in ancestral line, the Activin gene of guinea fowl and quail
cannot be compared with guinea fowl-turkey (95%) nor guinea fowl-chicken (90%),
in both biological functions and evolutionary relationship. Genetic diversity
is useful especially for selecting animals with high reproductive efficiency
and fertility potential which are most preferred by ranchers. Diversity in
activin gene may lead to variation in the expression of function with avians
having more of such variation. Finally, the percent identity and similarity in
function of ACTIVIN gene of guinea fowl, turkey, and chicken were in the range
of 93-100%, indicating that activin gene of avians possesses similar functions,
well conserved and are very effective in performing functions like increasing
FSH bindings, FSH-induced aromatization, improves wound healing and enhances
scar formation, regulates morphogenesis of branching organs, enhances ovarian
folliculogenesis. The study therefore, recommends farmers to select and breed
for activin gene in order to promote reproductive efficiency, thereby
barricading species extinction.
Author(s) Details:
C. M. Ikele,
Integrated Germline Biology Group Laboratory, Osaka University,
Japan.
I. S. Ahamba,
Department
of Animal Science and Technology, Northwest A and F University, China.
M. Egom,
Department of Animal Science, Faculty of Agriculture, University of
Nigeria, Nsukka, Nigeria.
C. U. Ekugba,
Nnamdi Azikiwe University, Awka, Nigeria.
C. E. Awaogu,
Faculty of Natural and Agricultural Sciences, Mafikeng Campus,
Northwest University, Mmabatho, South Africa.
Please see the link here: https://stm.bookpi.org/ARBS-V8/article/view/13288
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