Low concentrations of dopamine in the central nervous system
cause several neurological diseases, such as schizophrenia and Parkinson's
disease. A simple electrochemical sensor for dopamine detection, based on
molecularly imprinted and electropolymerized over-oxidized polypyrrole (OPPy)
is designed. It is based on the electropolymerization of pyrrole in the
presence of the template molecule, dopamine (DA). The square wave voltammetry
(SWV) is used for the detection of dopamine in a buffer solution. A molecularly
imprinted polymer (MIP) is formed in the presence of a target molecule and,
after extraction, a complementary cavity is formed, with a chemical affinity
for the target molecule which favors its adsorption. The current peak obtained
at the MIP electrode was proportional to the logarithm of the DA concentration
in the range of 10-11 to 5x10-8 M and a detection limit of 10-11 M is obtained.
The proposed sensor was used for the detection of DA in spiked blood serum,
satisfactory results were obtained, showing the possible application of this
dopamine sensor in biological fluids. MIP-OPPy has demonstrated high
sensitivity compared to published dopamine electrochemical sensors based on
polypyrole. This sensor can be easily manufactured at low cost and can be
applied for the determination of dopamine in human serum.
Author(s) Details:
Sarra Slimi,
Laboratory of Advanced Materials and Interfaces, Faculty of Sciences, University of Monastir, Monastir 5000, Tunisia.
Chama Mabrouk,
Laboratory of Advanced Materials and Interfaces, Faculty of Sciences, University of Monastir, Monastir 5000, Tunisia.
Houcine Barhoumi,
Laboratory of Advanced Materials and Interfaces, Faculty of Sciences, University of Monastir, Monastir 5000, Tunisia.
Nicole Jaffrezic,
Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, Lyon, France.
Please see the link here: https://stm.bookpi.org/CPPSR-V6/article/view/13248
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