In-silico and In-vitro Antimitotic Activity of Some Novel 6-Fluoro-1, 2, 4-Triazolo-Benzothiazole Analogues | Chapter 5 | Advanced Concepts in Pharmaceutical Research Vol. 5

Worldwide, cancer is a leading cause of death in both developed and developing countries. Numerous natural and manmade anticancer drugs treat solid tumors, lymphomas, and leukemias in different forms. In this work, nine 6-fluoro-triazolo-benzothiazole derivatives were prepared and evaluated for in vitro antimitotic activity. In addition, an in-silico study was also done using tubulin protein (PDB: 6QQN) by molecular docking method. Thin-layer chromatography (TLC) was used to monitor the progress of the reaction progress. Results revealed that TZ2 and TZ9 were the most active compounds with antimitotic action opposing the standard drug, aspirin. Results of molecular docking exhibited the inhibitory potential of triazole-benzothiazole against tubulin protein. The mitotic study indicates the efficacy of triazole-benzothiazole analogues in inhibiting the proliferation of cancer cells either by promoting microtubule formation or affecting microtubules, thereby preventing microtubule breakdown. The obtained results suggest that germinated mung beans may be a useful tool for quickly and affordably evaluating new medicines' cytotoxic effects.

Author(s) Details:

Naresh Podila,
Department of Pharmaceutical Sciences, Vignan's Foundation for Science, Technology and Research, Guntur, Andhra Pradesh, 522213, India.

Sanapala Arun Kumar,
Sree Dattha Institute of Pharmacy, Sheriguda, Ibrahimpatnam, Rangareddy, Telangana, 500001, India.

Vijaya Kishore K.,
Department of Pharmaceutical Sciences, College of Pharmaceutical Sciences, Acharya Nagarjuna University, Guntur, Andhra Pradesh, 522510, India.

Rajashekar V.,
Department of Pharmaceutical Chemistry, Anurag Pharmacy College, Ananthagiri, Kodad, Suryapet, Telangana, 508206, India.

Risy Namratha Jamullamudi,
Department of Pharmaceutical Regulatory Affairs, Siddhartha Institute of Pharmaceutical Sciences, Narasaraopeta, Andhra Pradesh, 522601, India.

Prasada Rao Manchineni,
Department of Pharmaceutical Analysis, Medarametla Anjamma Mastan Rao College of Pharmacy, Narasaraopet, Palnadu, Andhra Pradesh, 522601, India.

Namratha Sunkara,
Bharat Institute of Technology, Ibrahimpatnam, Rangareddy District, Telangana, 501506, India.

Sreelatha Muddisetti,
Vikas College of Pharmaceutical Sciences, Suryapet, Telangana, 508376, India.

Please see the link here: https://stm.bookpi.org/ACPR-V5/article/view/13245