The objective of this research was to develop a polyherbal
formulation using three different herbs and to evaluate their phytochemicals,
physical constants and determination of their antioxidant activity by DPPH
method. Folklore medicine is being re-evaluated by extensive research on
different plant species and their therapeutic principles. The PHF authenticated
herbs were characterized by studying their morphological and phytochemical
analysis. Preliminary screening showed the presence of alkaloids, glycosides,
carbohydrates, amino acids and flavonoids in the combination extract. Physical
parameters such as loss on drying (LOD), ash values and extractive values have
been studied. The antioxidant activity of the combination of extract (100 mg
each) was determined using DPPH free radical scavenging method. The results
showed that the combination extract has best antioxidant effect at a dose of
300 µg/ml and the % inhibition of PHF is 75.2% and 96.6 % inhibition for
ascorbic acid as the reference standard. All the extracts and PHF showed a dose
dependent activity. Fourier transform infrared spectroscopy (FTIR) uses the
mathematical process (Fourier transform) to translate the raw data
(interferogram) into the actual spectrum. FTIR method is used to obtain the
infrared spectrum of transmission or absorption of a fuel sample. The result showed that the PHF has more
potent antioxidant activity when compared with individual extract. It also
shows that the combination of three different herbs in a single formulation is
completely compatible with each other which is determined from FTIR. The
presence of heavy metal and minerals in the prepared extracts and PHF where
analysed using atomic absorption spectroscopy. The results showed that the
tested minerals and metals were within the specified limit. From the study, the levels of these metals
were detected in all three plants and PHF. Presence of heavy metals and
minerals in the prepared extracts and PHF were analysed using AAS and were with
in the specified limits.
Author(s) Details:
Segu Prathyusha,
School of Pharmacy, Guru Nanak Institutions Technical Campus,
Hyderabad, Ranga Reddy - 501506, India.
Ratnakar
Cherukupally,
School of
Pharmacy, Guru Nanak Institutions Technical Campus, Hyderabad, Ranga Reddy -
501506, India.
Saraswathi Kaspa,
School of Pharmacy, Guru Nanak Institutions Technical Campus,
Hyderabad, Ranga Reddy - 501506, India.
Sumalatha K.,
School of Pharmacy, Guru Nanak Institutions Technical Campus,
Hyderabad, Ranga Reddy - 501506, India.
Shaik Harun Rasheed,
School of Pharmacy, Guru Nanak Institutions Technical Campus,
Hyderabad, Ranga Reddy - 501506, India.
Ch. Pavani,
School of
Pharmacy, Guru Nanak Institutions Technical Campus, Hyderabad, Ranga Reddy -
501506, India.
Please see the link here: https://stm.bookpi.org/ACPR-V5/article/view/13244
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