Identification of Molecular Characteristics of Babesia spp. and Anaplasma marginale in Water Buffaloes: A Retrospective Study from Veracruz and Tabasco, Mexico | Chapter 3 | Research Advances in Microbiology and Biotechnology Vol. 3

 The main objective of the study was to discover, with PCR assays and ratify by DNA sequencing of amplified aim genes, the presence of Babesia and Anaplasma sp. in samples from polluted water buffaloes in Veracruz and Tabasco, Mexico. Babesiosis, theileriosis and anaplasmosis are important tick-carried diseases of oxen worldwide containing Kenya. Babesiosis and theileriosis are caused apiece protozoan freeloaders, Babesia and Theileria respectively while anaplasmosis is generated by rickettsial organism popular as Anaplasma. A cross-sectional study was administered in 2017, using a non-probabilistic inspecting method for availability. The total population of water buffaloes from two result units (PU), situated at the municipality of Sayula de Aleman, state of Veracruz, Mexico, were sampled for this study: Veracruz 1 (n = 61) and Veracruz 2 (n = 63). Similarly, the total people of water buffaloes from two production parts (PU), in the municipality of Villahermosa, Tabasco state, Mexico, was sampled for this study: Tabasco 1 (n = 58) and Tabasco 2 (n = 51), for a dignified total of 233 water buffalo samples. Specific genes encrypting the B. bovis rhoptry-associated protein (RAP-1), the B. bigemina SpeI-AvaI limit fragment, and the Anaplasma marginale major surface protein 5 (MSP5) were exaggerated using reside PCR assays. The identities of the hemoparasites were established by equating the nucleotide sequences obtained in this place study with those possible in the GenBank database at the National Center for Biotechnology Information (NCBI) utilizing DNA sequencing and BLASTn analysis for DNA similarity hemoparasite identification. Water confuse infection accompanying at least individual of the hemoparasites under study was discovered in 45% (105/233) of the blood samples, while a assorted infection accompanying B. bovis and B. bigemina was detected in 6.4% (15/233) of samples. For this cross-localized study, mixed contaminations with the three hemoparasites were not discovered. BLASTn analysis disclosed that the nucleotide sequences of the water buffalo isolates joint sequence similarity values ranging from 88 to 100% accompanying previously written gene sequences of B. bovis, B. bigemina, and A. marginale. The judgments of this study manifest that water buffalo are tick-carried hemoparasite carriers, just like oxen, and that they likely play a important role in the community health of bovine babesiosis in Mexico.

Author(s) Details:

José Juan Lira-Amaya,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Rebeca Montserrat Santamaria-Espinosa,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Roberto O. Castañeda-Arriola,
Sitio Experimental Pichucalco, CIRGOC, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Km. 8, Carretera Pichucalco-Teapa, Pichucalco, 86040, México.

Grecia Martínez-García,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Diego J. Polanco-Martínez,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Carmen Rojas-Martínez,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Jesús Ántonio Álvarez-Martínez,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Julio V. Figueroa-Millán,
CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera Federal Cuernavaca Cuautla No. 8534, Colonia Progreso, Jiutepec, 62550, México.

Please see the link here: https://stm.bookpi.org/RAMB-V3/article/view/9761