This paper describes the growth and plasmid stability of the E. coli JM109 host system. Plasmids are crucial tools for biotechnology, and for better industrial applications, a solid grasp of their biology is essential. The host system was exposed to a variety of pharmaceutical and chemical concentrations, as well as radiation frequencies, to verify the colony forming units, the concentration, and the stability of plasmids. The chemical acridine orange had the biggest effect on DH5a's growth, whereas the drug dantron had the biggest effect on the organism's development. Microwave radiation at 2GHz and low intensity was found to have the biggest inhibitory effects. However, there was no discernible effect of UV exposure on growth. According to the results of the current study, plasmid concentration and stability are significantly impacted by medications, chemicals, radio frequency, and microwave radiation in addition to organism growth.
Author(s) Details:
Praveen Kumar Vemuri,
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Shaik Mohammad Anjum,
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Krishna Keerthika Oruganti,
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Sarvani Gadiraju,
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Yeswanth Sai Kumar Thota,
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Andhra Pradesh, India.
Please see the link here: https://stm.bookpi.org/RABS-V9/article/view/8342
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