Multiplex SYBR Green Real-Time PCR Assay for Detection of Common Respiratory Pathogens of Poultry| Chapter 7 | Newest Updates in Agriculture and Veterinary Science Vol. 1

 Due to exhaustive farming practises and coinfection accompanying bacteria (MG, MS, E. coli, and Avibacterium spp.) and viruses (IBV and ILTV), assorted respiratory infections are extensive in poultry.The normal methods are difficult and time-consuming, so this study was attended with the following aims in mind to address the challenges associated with the discovery of mixed respiratory pathogens: Multiplex SYBR Green Real-Time PCR assay for discovery of common respiring pathogens of poultry by address IGSR gene for MG, vlhAgene for MS, N deoxyribonucleic acid for IBV and ICP4 gene for ILTV in alone tube. The amended conditions for all genes was initial denaturation at 94oC for 3.59 mins, attended by 40 cycles of denaturation at 94oC for 20 seconds, annealing at 55oC for 45 seconds and enlargement at 72oC for 1 min and conclusive extension step was at 72oC for 10 mins.  The amplifications of all four genes was noticed and recorded the ct principles of 30.28 (IGSR gene), 24.16 (vlhA deoxyribonucleic acid), 19.39 (ICP4 gene) and 11.81 (N gene) and still the recorded Tms were    74.0 (IGSR deoxyribonucleic acid), 82.5 (vlhA gene), 85.5 (ICP4 deoxyribonucleic acid) and 80.5 (N gene). The samples were regarded positive if the written threshold phase value was lower than 35. 19 farms were restrained, and 2 tested certain for ILT, 10 for MG, 7 for MS, and 5 for both MG and MS. All 19 proven negative for IB. In  screened samples, the written ct values  for IGSR genes categorized between 11.81 to 30.28, product Tms categorized  between 74 to 87, for vlhA deoxyribonucleic acid ct values categorized between 17.39 to25.81, brand Tms ranged 'tween 81 to 87, for ICP4 gene ct advantage  was 19.08 and 19.23,product Tms was 81 and 80.5. Rapid, accurate and concurrent detection of the respiring pathogens and adoption of appropriate control plans in the poultry farms take care of greatly help in fighting the economic misfortunes in the poultry industry

Author(s) Details:

Nagendra Reddy Thopireddy,
Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502,India.

Sreedevi Bollini,
Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502,India.

Vinod Kumar Nagaram,
Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-517502,India.


Please see the link here: https://stm.bookpi.org/NUAVS-V1/article/view/8560