Strong Anti-inflammatory Effect of Soluble Curcumin Shown at Cellular Level | Chapter 11 | Current Progress in Medicine and Medical Research Vol. 8

 This phase aims to explore potential of a suitable drug molecule that can symbolize an ideal candidate for bringing to bear its therapeutic conduct in order to cure or manage pathological conditions at instigative levels. Inflammatory mechanisms have combine of the strongest underlying creative factors private of the diseases. Atherosclerosis is individual such inflammatory synopsis wherein Endothelial container (EC) activation and chronic swelling can cause the initiation and progress of the disease. A promising competitor medicine accompanying few negative effects is curcumin (CM). However, its healing usage is forced by poor water solubility, a lack of bioavailability, and important hydrolytic breakdown. Conjugation of CM to Albumin (Alb) producing CM-Alb increases the solubility of the potential drug in addition to targeted drug transfer with negligible antagonistic effects from the drug one who carries or transmits something. This study evaluates an albumin-CM conjugate for cellular rude answer demonstrating bioavailability, deciding the non-cytotoxic concentrations and anti-inflammatory answer in human in the middle vein endothelial cells (HUVECs). The maximum indulged biocompatible dose driven in HUVEC (EC) culture is 30µM; therefore, this study evaluates an anti-inflammatory answer at non-toxic concentrations. The experiments utilizing fluorescence microscopy and flow cytometry confirmed the endocytosis of glowing-tagged CM-Alb and granted comparable cellular rude answer of native albumin. Cytokine- induced incitement of inflammation in EC culture was patterned as in vitro model for judging the response to CM-Alb. A important increase in the expression of VCAM-1 in EC upon incubating accompanying TNF-α for a period of 24h gives a good model method to study the effect of the anti-inflammatory drug particle. Further, the treatment of swelling-induced ECs with CM-Alb combine at 5, 10, 20µM concentrations down-controlled the expression of VCAM-1 significantly distinguished to the TNF-α treated containers. Expressions of markers at a protein level in cytokine-mobilized EC drop down to normalcy when doctored with CM-Alb. In the swelling-induced culture example, the improved liquid solubility and receptor-mediated absorption of the CM- Alb compound by ECs developed in an anti-instigative response, suggesting the possibility for further research as an drip medicinal expression.

Author(s) Details:

Deepa Sathee,
Division of Thrombosis Research, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.

Lissy Kalliyana Krishnan,
Division of Thrombosis Research, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.

Please see the link here: https://stm.bookpi.org/CPMMR-V8/article/view/11742