A new arrangement was established for concurrent estimation of Ivermectin and Albendazole by RP-HPLC method. The chromatographic environments were successfully grown for the separation of Ivermectin and Albendazole by utilizing ACE C18 column (4.6×150 mm) 5µ, flow rate was 1.2 ml/min, travelling phase percentage was (70:30 v/v) methanol: Phosphate safeguard pH 3 (pH was adjusted with ortho phosphoricacid), discovery wavelength was 240 nm. The means used was WATERS HPLC Auto Sampler, Separation piece 2690, photodiode array detector 996, Empower-software variant-2. The retention periods were found expected 2.344 mins and 3.284 mins. The % purity of Ivermectin and Albendazole was found expected 101.27% and 99.97% respectively. The system rightness parameters for Ivermectin and Albendazole such as hypothetical plates and tailing determinant were found expected 4668, 1.3 and 6089 and 1.2, the resolution was found expected 6.0. The analytical means was validated in accordance with ICH guidelines (ICH, Q2 (R1)). The linearity study n Ivermectin and Albendazole was in the direction of concentration range of 50 µg-250 µg and 5µg-50µg and equivalence coefficient (r2) was erect to be 0.999 and 0.999, % recovery was erect to be 99.56% and 99.48%, % RSD for repeatability was0.2 and 0.2, % RSD for middle precision was 0.2 and 0.1respectively. The accuracy study was precise, robust, and repeatable. LOD profit was 3.17 and 5.68, and LOQ value was 0.0172 and 0.2125 individually. Hence the suggested RP-HPLC pattern can be used for routine reasoning of Ivermectin and Albendazole in API and Pharmaceutical dosage form.
Author(s) Details:
Meruva Sathish Kumar,
MNR
College of Pharmacy, Fasalwadi, Sangareddy, India.
Phani
Deepika Polampalli,
MNR
College of Pharmacy, Fasalwadi, Sangareddy, India.
Bandi Narendar,
MNR College of Pharmacy, Fasalwadi, Sangareddy, India.
S. Siva Prasad,
MNR College of Pharmacy, Fasalwadi, Sangareddy, India.
Rasapally Ramesh Kumar,
MLR
College of Pharmacy, Dundigal, India.
Please see the link here: https://stm.bookpi.org/NACB-V7/article/view/11964
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