Micro-Propagation Methodology for the Apple Balady Cultivar Utilizing Leaf Explants | Chapter 7 | Emerging Issues in Agricultural Sciences Vol. 7

 The goals of this study search out establish a fabric culture and rapid calculating-propagation concerning details system, in addition to an efficient shoots conversion method appropriating leaf explants from the Balady apple genotype. The belongings of various types and amounts of cytokinin and carbon beginnings on shoots regeneration were intentional using artificial leaves as explants for adventitious shoots initiation. The optimal medium for encouraging callus from leaf explants was MS medium holding 2.0 mg/l BA and 5.0 mg/l NAA. MS medium supplemented with 3.0 mg/l BA + 1.0 mg/l Kin + 0.5 mg/l NAA were the optimum medium for apple shoots distinction and proliferation. MS medium reinforced with 2.0 mg/l TDZ and 0.1 mg/l NAA was best choice medium for shoots regeneration, bearing the greatest number of shoots (20.32 shoots /jar). The adding of 30 g/l of Sorbitol to the culture medium was the most productive for in vitro shoots increase. With a combination of 2.0 mg/l GA3 and 3.0 mg/l BA, ultimate strong development and elongation were realized. The medium with the highest ingraining rate of 87.6% was ½ MS with 1.5 mg/l IBA additional.

Author(s) Details:

Sayed, A. A. Elsayh,
Central Lab of Date Palm Researches and Development, Agricultural Research Center, Giza, Egypt.

Rasha, N. Arafa,
Horticulture Research Institute, Breeding Research Department, Agricultural Research Center, Giza, Egypt.

Eman H. Afifi,
Central Lab of Date Palm Researches and Development, Agricultural Research Center, Giza, Egypt.

Marwa M. Abdalgaleel,
Horticulture Research Institute, Breeding Research Department, Agricultural Research Center, Giza, Egypt.

Alaa N. Draz,
Agriculture Botany-Department, Genetics, Faculty of Agriculture, Al Azhar University, Cairo, Egypt.

Salwa El-Habashy,
Horticulture Research Institute, Breeding Research Department, Agricultural Research Center, Giza, Egypt.

Emadeldin A. H. Ahmed,
Horticulture Research Institute, Breeding Research Department, Agricultural Research Center, Giza, Egypt.

Please see the link here: https://stm.bookpi.org/EIAS-V7/article/view/11852