Increased intracellular water content, also known as cytotoxic brain tissue edoema, is a significant subsequent clinical consequence of TBI and stroke that occurs without awareness of the aetiology. Recently, a theory for nervous tissue edoema was proposed, claiming that protein unfolding was triggered by external dynamic and internal mechanical static impact forces, resulting in an increase in brain tissue water content. Computer simulation testing backed up the hypothesis. In this laboratory investigation, we tested the concept further by examining the effects of both dynamic and static impact forces on the mature protein laminin LN521. Because of its widespread and abundant presence in cells, laminin was chosen as a representative protein. Denatured electrophoresis and electron microscopy were used to examine the treated laminin solutions, which revealed aggregation and fragmentation of the laminin structures. The current findings back up an earlier idea and computer simulation, indicating for the first time that dynamic impact force in an accident and increased mechanical static force in a stroke can cause mature proteins to unfold, potentially causing cytotoxic brain tissue edoema. The clinical situation is similar to what happens in the deep water when elasmobranchs, such as white sharks, protect their cells from too much hydrostatic pressure. As a result, the findings of the current laboratory investigation, as well as knowledge from marine physics, could be used to improve clinical treatment and outcomes for TBI and stroke patients.
Author (S) Details
Hans von Holst
Section of Neurosurgery, Karolinska University Hospital, Sweden and Section of Research, MIPS Company, Sweden.
Pasi Purhonen
Department of Biosciences and Nutrition, Karolinska Institute and School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Huddinge, Sweden.
Daniel Lanner
Section of Research, MIPS Company, Sweden.
Ramakrishnan Balakrishnan Kumar
Department of Biosciences and Nutrition, Karolinska Institute and School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Huddinge, Sweden.
Hans Hebert
Department of Biosciences and Nutrition, Karolinska Institute and School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Huddinge, Sweden.
View Book :- https://stm.bookpi.org/NFMMR-V15/article/view/3866
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