Role of PKC in CD47-Mediated Phosphatidylserine Expression Pathway in Jurkat T Cells: A Preliminary Study | Chapter 10 | Current Aspects in Pharmaceutical Research and Development Vol. 2

 T-cell acute lymphoblastic leukaemia (T-ALL) treatment failure occurs when leukemic blasts develop resistance to chemotherapeutic drugs. The hunt for targets for the creation of more effective and less toxic anti-leukemic medicines is now underway. CD47 has been linked to chemo-resistance and cancer cell metastasis. Although numerous possible pathways have been proposed to explain the therapeutic impact of CD47-targeting, the downstream effectors that contribute to varied CD47 effects are yet unknown. We investigated the involvement of Protein Kinase C (PKC) in the CD47-mediated phosphatidylserine expression pathway in Jurkat T cells in this early work. In the presence or absence of Bisindolylmaleimide I, hydrochloride, Jurkat T cells were incubated with anti-CD47 mAb (BRIC 126), anti-CD44 mAb (BRIC 235), or control (PKC inhibitor). Annexin-V FITC was used to label the cells. Fluorescence intensity was measured using flow cytometry analysis. The trypan blue exclusion test was used to determine cell viability. In the CD47 receptor mediated leukaemia cells apoptotic pathway, inhibiting PKC increased phosphatidylserine expression. This suggests that PKC is involved in the PS exposure route in jurkat T cells mediated by CD47. CD47 and PKC were discovered to be novel functional proteins in jurkat T cells with intriguing therapeutic potential, according to the findings of this study. This research could lead to new approaches to treating T-ALL illness.

Author(s) Details

Ikenna Kingsley Uchendu
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Ogbonna Kosisochukwu Doris
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Oguji Cyprian Emeka
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Omeh Johnson Obiechina
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Ochi John Chidubem
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Udeh Abel Chukwuebuka
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Obigeorge Chibueze Joseph
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Chinecherem Maudlyne Nnam
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

Olatunji Omokungbe
Department of Medical Laboratory Science, University of Nigeria, Enugu Campus, Enugu State, Nigeria.

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