Pigment Production in Callus Culture of ‘Karandafi’ Red Sorghum (Sorghum bicolor (L.) Moench). A Probable Cure for Sickle Cell Anaemia | Chapter 4 | Recent Research Advances in Biology Vol. 8

 ‘Karandafi' is a sorghum landrace having antisickling properties and high levels of 3-deoxyanthocyanidin, however it is mutant in nature. This tends to impede large-scale manufacture of this key medication for sickle cell anaemia treatment. Sorghum bicolor is high in phytochemicals, and its extract is used to treat sickle cell anaemia in combination with other medicinal plants. The goal of this research is to see how varied hormone concentrations, alone or in combination with an elicitor, affect pigment production in Karandafi in vitro. For callus induction using mature seeds as explant, Murashige and Skoog (MS) basal media supplemented with different concentrations of 2, 4-D (0, 2 and 4 mg/L) alone or in combination with 0.5 mg/L NAA or KN was used, while for pigment production MS media supplemented with BAP (0-3 mg/L) alone or in combination with 1 mg/L KN, NAA, or 50 mg/L yeast extract (Y.E) was used. Percentage callus development, callus fresh weight, callus induced nature, percent shoot and root formation, number of roots, shoot and root length were all collected. All of the traits studied showed a highly significant difference. The best hormone doses for callus induction are 2 mg/L 2,4-D alone or in combination with 0.5 mg/L NAA and KN, whereas greater callus growth was achieved in the media fortified with 4 mg/L 2,4-D +0.5 mg/LKN (3.43 g). In Karandafi, pigment synthesis was seen in conditions supplemented with BAP alone or in combination with NAA, KN, or yeast extract, however extraction and quantification revealed the optimal treatment with the highest level of 3-deoxyanthocyanindin.

Author (s) Details

L. B. Hassan
Department of Plant Science, Faculty of Agriculture, Ahmadu Bello University, Nigeria.

I. S. Usman
Department of Plant Science, Faculty of Agriculture, Ahmadu Bello University, Nigeria.

A. Usman
Department of Plant Science, Faculty of Agriculture, Ahmadu Bello University, Nigeria.

H. Ibrahim
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Nigeria.

H. O. Ahmed
Department of Plant Science, Faculty of Agriculture, Ahmadu Bello University, Nigeria.

S. Mohammed
Department of Plant Science, Faculty of Agriculture, Ahmadu Bello University, Nigeria.

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