Effects of Methylmercury and Theaflavin Digallate on Adipokines in Mature 3T3-L1 Adipocytes: A Recent Study | Chapter 3 | Current Advances in Chemistry and Biochemistry Vol. 6

 Diabetes is a leading cause of morbidity around the world, and it is frequently associated with obesity, metabolic syndrome, and other inflammatory diseases associated with aging. Environmental factors such as metals and persistent organic pollutants, in addition to genetic and lifestyle factors, may increase the severity or lower the threshold of these conditions. Methylmercury is toxic to adipocytes in cell culture and may affect adipokine secretion. The effects of different concentrations of theaflavin digallate on methylmercury-exposed 3T3-L1 adipocytes in cell culture were investigated in this study. Resistin, adiponectin, and the lipid peroxidation product 4-hydroxynonenal (4-HNE) were all secreted. Lipid (Oil Red O) staining was used to evaluate the treatment of adipocytes. When compared to control cells, methylmercury exposure increased the levels of resistin, adiponectin, and 4-HNE. Cells treated with methylmercury had fewer adipocytes and clumped lipid droplets. These findings suggest that methylmercury causes reactive oxygen species to be produced, resulting in the development of an inflammatory response. Methylmercury was reduced by theaflavin-3,3′-digallate. maintaining adipocyte morphology and adiponectin, resistin, and 4-hydroxynonenal secretion patterns Other anti-inflammatory and signaling agents could be tested at the biochemical level using this experimental model system before being studied in animal models. To gain a better understanding of the anti-oxidative effects of polyphenolic compounds, different TF-3 concentrations can be used.

Authoor (S) Details

Shubhangi Chauhan
Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK 99775-6160, USA and Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7000, USA.

Kriya Dunlap
Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK 99775-6160, USA and Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7000, USA.

Lawrence K. Duffy
Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK 99775-6160, USA and Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7000, USA.

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