The ability of bacterial strains Rhodococcus sp. S, Pseudomonas sp. S, and Consortium M isolated from hydrocarbon contaminated sites in Skikda (Algeria) and Alexandria (Egypt) to bioremediate crude oil contaminated sea water in lab microcosms was tested. Various treatments, such as bioaugmentation, biostimulation, and attenuation, were tried. As a nitrogen source, ammonium nitrate was used. Phosphorus was obtained from disodium hydrogen phosphate and sodium dihydrogen phosphate. Except for the attenuation treatment, crude oil degradation after six weeks ranged between 70.5 and 95 percent (55.5 percent ). When compared to free cells, wheat straw immobilized cells improved crude oil degradation. After four weeks, immobilized Rhodococcus sp. S and Consortium M cells degraded 88 and 91 percent of crude oil, respectively, in the presence of Triton X-100. In the absence of Triton X-100, they could degrade by 89.5 and 90%, respectively. The scaling-up of lab microcosms revealed that bioaugmentation with immobilized Consortium M cells produced the best results. The increase in crude oil biodegradation was associated with an increase in dehydrogenase activity. The toxicity of crude oil was studied using local marine algae. The results showed that bioremediated sea water was less toxic than untreated sea water.
Author (S) Details
Zohra Chekroud
Laboratory of Interactions of Biodiversity, Ecosystems and Biotechnology, University of 20th August 1955, Skikda, Algeria
Mona Khamees Gouda
Department of Botanic, Faculty of Science, Alexandria University, Egypt.
Moussa Houhamdi
Department of Biology, Faculty of Sciences of Nature, of Life, of Earth and of the Universe, University of 8th Mai 1945, Guelma, Algeria.
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