Determination of Bacteriological Profile of Nosocomial Infections in Visceral Surgery at CNHU-HKM, Republic of Benin | Chapter 12 |Innovations in Microbiology and Biotechnology Vol.4

 The goal of this study is to identify the bacteria that cause these suppurations, investigate their antibiotic resistance profiles, and assess the role of the nurses who care for these patients. The production and buildup of pus in the soft tissue of a wound is known as wound suppuration. In most cases, the normal inflammatory reaction subsides after three to five days, and the wound heals, causing pus to accumulate, slowing the healing process and potentially leading to significant repercussions. From May 15 to June 21, 2013, the research was carried out at the Menontin zone hospital. Fifty-one patients of all ages and genders with surgical and traumatic discharge wounds were sampled, as well as 30 hand samples from nurses' hands before and after dressing. 76 microorganisms were detected from the results, comprising 35 cocci and 41 bacilli. Pseudomonas aeruginosa was detected in 29% of the microorganisms, Staphylococcus aureus in 28%, Escherichia coli in 14%, and miscellaneous bacteria in 14%. 10 bacteria were identified from hand samples, including 4 Pseudomonas aeruginosa and 3 Staphylococcus DNase-negative. 1 Staphylococcus aureus and 2 Klebsiella spp. The isolates were highly resistant to the antibiotics tested, particularly -lactamβ, Gentamycin, Erythromycin, and Colistin. Ciprofloxacin and Fosfomycin sensitivity is high in the majority of strains. It was determined that patients and nurses should be aware of the presence of these germs, take proper antibiotics, ideally after an antibiotic test, wash their hands often, and use sterile materials before and after any dressing.

Author(s) Details:

Aïkou Nicolas,
Department of Human Biology, National University of Sciences, Engineering and Mathematics, Laboratory of Clinical Biochemistry and Medical Microbiology, Benin.

A. Ahoyo Theodora,
Abomey Calavi University, General Medical Microbiology and Hospital Hygiene Abomey Calavi Politechnical School, Human Biology Engineering, Benin.

Degbe Sah Cyriaque,
Regional Institute of Public Health Ouidah, Benin.

Coulibaly Founzégué Amadou,
Pasteur Institute Abidjan, Cote-d’ivoire.


Sezan Alphonse,
FAST/UAC, Benin.


Edorh A. Patrick,
FAST/UAC, Benin.


R. A. Lapo,
Inter-State School of Science and Veterinary Medicine, Dakar, Senegal.

Please see the link here: https://stm.bookpi.org/IMB-V4/article/view/6267

Bioremediation of Crude Oil Contaminated Sea Water | Chapter 7 | Current Advances in Chemistry and Biochemistry Vol. 6

 The ability of bacterial strains Rhodococcus sp. S, Pseudomonas sp. S, and Consortium M isolated from hydrocarbon contaminated sites in Skikda (Algeria) and Alexandria (Egypt) to bioremediate crude oil contaminated sea water in lab microcosms was tested. Various treatments, such as bioaugmentation, biostimulation, and attenuation, were tried. As a nitrogen source, ammonium nitrate was used. Phosphorus was obtained from disodium hydrogen phosphate and sodium dihydrogen phosphate. Except for the attenuation treatment, crude oil degradation after six weeks ranged between 70.5 and 95 percent (55.5 percent ). When compared to free cells, wheat straw immobilized cells improved crude oil degradation. After four weeks, immobilized Rhodococcus sp. S and Consortium M cells degraded 88 and 91 percent of crude oil, respectively, in the presence of Triton X-100. In the absence of Triton X-100, they could degrade by 89.5 and 90%, respectively. The scaling-up of lab microcosms revealed that bioaugmentation with immobilized Consortium M cells produced the best results. The increase in crude oil biodegradation was associated with an increase in dehydrogenase activity. The toxicity of crude oil was studied using local marine algae. The results showed that bioremediated sea water was less toxic than untreated sea water.

Author (S) Details

Zohra Chekroud
Laboratory of Interactions of Biodiversity, Ecosystems and Biotechnology, University of 20th August 1955, Skikda, Algeria

Mona Khamees Gouda
Department of Botanic, Faculty of Science, Alexandria University, Egypt.

Moussa Houhamdi
Department of Biology, Faculty of Sciences of Nature, of Life, of Earth and of the Universe, University of 8th Mai 1945, Guelma, Algeria.

View Book :- https://stm.bookpi.org/CACB-V6/article/view/1439