Unfavorable environmental conditions cause biofilm formation by altering the expression of several genes. Biofilm, in turn, alters the microenvironment of its inhabitants, resulting in changes in gene expression and biofilm maturation. Stenotrophomonas maltophilia is a Gram-negative, aerobic, glucose-nonfermentative bacterium that is widely distributed in the environment. The current study describes the isolation, identification, genotypic and phenotypic characterization of Stenotrophomonas strains isolated from industrial effluent samples, as well as the ability of Stenotrophomonas maltophiliaSRS05- GRD (KF558319) for biofilm formation using different substrates and different environments. Glass was found to support high biofilm formation of Stenotrophomonas maltophilia SRS05-GRD based on a graphical representation of optical density values obtained by incorporating various substrates in Tryptone Soya Broth (TSB). The results show that the commercial media (TSB with glucose and soybean meal at pH 7) promotes biofilm formation the best. As a result of this study, the ability of Stenotrophomonas maltophilia SRS05-GRD to form biofilm has been confirmed. The optimal culture medium and dilution were also determined, which resulted in effective biofilm formation.
Author(s) Details
Department of Biotechnology, Dr. G. R. Damodaran College of science, Coimbatore-641 014, Tamil Nadu, India.
M. S. Jebil
Department of Biotechnology, Dr. G. R. Damodaran College of science, Coimbatore-641 014, Tamil Nadu, India.
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