Objective: Alpinia calcarata Roscoe (Family: Zingiberaceae) rhizomes are frequently used in Indian usual systems of cure as a remedy for bronchitis, cough, respiratory illnesses, diabetes mellitus, asthma and arthritis. The Present study was designed to examine the anit arthritic feature of Stigmasterol isolated from Alpinia calcarata rhizomes of methanolic extract by In-vitro studies.Methods: As per International Conference on Harmonization (ICH) directions we have used different concentrations of Stigmasterol as standard on HPTLC plates for the calculation of Stigmasterol from the Alpinia calcarata rhizomes and the concentration of standard Stigmasterol was 1mg/ml. Before that it was freed by HPLC method and from UV, FTIR, 1H and 13C NMR and Mass spectroscopic methods. It was judged by in-vitro anti hard activities utilizing percentage of protein denaturation and HRBC sheath stabilization order.Results: The retention determinant of Stigmasterol was 0.58. Linearity was obtained in the range of 50 mg - 250 mg for Stigmasterol. The grown and validated HPTLC system was employed for Stigmasterol in methanolic rhizomes extract of Alpinia calcarata for uniformity of the content of the stone. The linear reversion data for the measurement plots showed a good uninterrupted relationshipwith r=0.99977 for Stigmasterol, respectively Satisfactory recoveries of 99.77% were got for Stigmasterol. UV, FTIR and NMR values were unusually correlated accompanying systhesised stigmasterol. It shows 99.4% of protein denaturation at 500 μg/mL. The isolated compound shows greater activity accompanying the IC50 values of 280.1 μg/mL than standard Diclofenac sodium accompanying the IC50 value of 298.34 μg/mL individually. It shows 75.59% of HRBC membrane counterweight at 500 μg/mL. The unique compound stigmasterol shows higher exercise with the IC50 principles of 355.71 than standard Diclofenac sodium with the IC50 worth of 351.15 μg/mL respectively. The unique compound stigmasterol displayed extraordinary anti inflexible activity.Conclusion: The results got in validation signify the accuracy and dependability of the developed HPTLC design for the quantification of Stigmasterol in methanolic rhizomes extract of Alpinia calcarata. The description of stigmasterol confirm the form and allure biological studies show that it is an anti-crippled agent for the first occasion.
Author(s) Details:
P. Pratheema,
PG and Research Department of Chemistry, Holy
Cross College (Autonomous), Tiruchirappalli, India.
L.
Cathrine,
PG
and Research Department of Chemistry, Holy Cross College (Autonomous),
Tiruchirappalli, India.
Please see the link here: https://stm.bookpi.org/COPS-V7/article/view/9698
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