Marine crabs are a rich source of bioactive chemicals with pharmacological capabilities, and antibacterial efficacy against clinically important infections has been established. The antibacterial activity of solvent extracts of different tissues and hemolymph lectin of Atergatis integerrimus against four fish and human pathogenic bacteria and four fungal pathogens is investigated in this study. E. coli was sensitive to the majority of the solvent extracts, while Enterococcus faecalis was particularly sensitive to the chloroform extract of the carapace and the butanol extract of the hemolymph. With butanol extract of entire body against Streptococcus mutans and Bacillus subtilis, the maximum zone of inhibition was recorded. The butanol extract of ovary, whole body, and crude hemolymph showed the greatest zone of inhibition against Vibrio harveyi when evaluated against the diverse tissue extracts. The acetone and ethylacetate extracts of hepatopancreas were toxic to Aeromonas hydrophila. The growth of Aspergillus flavus and Aspergillus niger was suppressed by butanol extracts from various tissues. Candida albicans growth was inhibited to considerable extent by chloroform, ethyl acetate, and butanol extracts. Almost all of the tissue extracts tested were resistant to Pencillium notatum. Based on preliminary research, a small number of pathogenic bacteria and fungi were chosen to investigate the effect of the pure lectin of Atergatis integerrimus, and antimicrobial activity was observed in all pathogens tested at a concentration of 100 g. As a result of our research, we can conclude that the tissue extract contains bioactive chemicals that particularly target pathogenic microorganisms, and that the isolated lectin, a crab defence molecule, has antimicrobial properties as well.
Author(S) Details
Katia Mareschi
Department of Public Health and Paediatrics, University of Turin, Piazza Polonia 94, 10126 Torino, Italy and Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
Sara Castiglia
Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
Aloe Adamini
Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
Deborah Rustichelli
Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
Elena Marini
Department of Public Health and Paediatrics, University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
Alessia Giovanna Santa Banche Niclot
Department of Public Health and Paediatrics, University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
Massimiliano Bergallo
Department of Public Health and Paediatrics, University of Turin, Piazza Polonia 94, 10126 Torino, Italy.
Luciana Labanca
Blood Component Production and Validation Center, City of Health and Science of Turin, S. Anna Hospital, 10126, Turin, Italy.
Ivana Ferrero
Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
Franca Fagioli
Department of Public Health and Paediatrics, University of Turin, Piazza Polonia 94, 10126 Torino, Italy and Stem Cell Transplantation and Cellular Therapy Laboratory, Paediatric Onco-Haematology Division, Regina Margherita Children’s Hospital, City of Health and Science of Turin, 10126, Torino, Italy.
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