Plant biotechnology is required to increase the chances of crop enhancement projects succeeding. DNA marker technology, which is derived from molecular genetics and genomics research, holds a lot of potential for plant breeding. In plant breeding, marker-assisted selection improves efficiency and precision. Mapping and marker-assisted selection of a certain characteristic necessitates high-quality DNA from a large number of plants in a short amount of time. Many techniques for extracting plant DNA have been developed and described, with the primary goal of developing a relatively rapid, inexpensive, and repeatable way to extract good quality DNA. All of these DNA extraction protocols follow the same general principle of disrupting the cell wall, cell membrane, and nuclear membrane to release highly intact DNA into solution while ensuring that contaminating biomolecules such as proteins, polysaccharides, lipids, phenols, and other secondary metabolites are removed using enzymatic or chemical methods. For marker assisted selection applications in crop improvement as well as sequencing-based applications, high quality DNA is a must. Plant Genomic DNA isolation and quantification procedures for several crops were included in this article.
Author(S) Details
Sushma Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior, Madhya Pradesh, India.
M. K. Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior, Madhya Pradesh, India.
R. S. Tomar
Rani Laxmibai Central Agricultural University, Jhansi, India.
Ashok Ahuja
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior, Madhya Pradesh, India.
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