Cordia dichotoma is the goal. The Boraginaceae family includes G. Forst, which is well-known for its biological qualities. The goal of this work is to look into the antibacterial activity of stem bark hexane extract by GC-MS analysis of its phytoconstituents.
The objectives include extracting the crude from plant bark using hexane as a solvent and determining the presence of bioactive chemicals in the hexane crude using the GC-MS technique. Column chromatography and preparative HPLC were used to purify the crude hexane extract. Further GC-MS analysis to determine the active chemicals contained in the purified fractions. -Sitosterol and Stigmasterol are isolated. The antibacterial activity of hexane crude was tested.
Methods: Cold and hot hexane solvents were used to extract the powdered stem bark, and the solvent from the extract was distilled off to be analysed by GC-MS. To purify the crude hexane further, saponification, column chromatography, and preparative TLC were utilised. GC-MS was used to analyse the fractions obtained using the methods outlined above. The antibacterial activity of the hexane extract was tested using the disc diffusion method against two bacterial pathogens.
GC-MS analysis identified eleven components from crude hexane extract, eight components from Fraction 16c, and three components from Fraction 16d based on peak area percentage, retention time, and structure.Author(S) Details
Gone Swapna
Department of Chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India.
Bhattiprolu Kesava Rao
Department of Chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India.
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