Wasabi contains a chemical called 6-Methylsulfinyhexyl isothiocyanate (6-MSITC) (Wasabia japonica Matsumura). In quiescent JB6 cells, 6-MSITC inhibited cell cycle progression when activated with epidermal growth factor. The goal of this work is to look into the mechanism of 6-MSITC's inhibitory effects on cell cycle progression induced by foetal bovine serum (FBS). In serum-starved JB6 cells for 36 hours, 6-MSITC reduced FBS-stimulated cell cycle advancement. Treatment with 6-MSITC, on the other hand, had no effect on Rb protein, cyclin D1, cyclin D2, cyclin D3, CDK4, CDK6, p16, p21, and p27, as well as cell growth signalling via the Akt and MAPK pathways. 6-MSITC, on the other hand, reduced DNA polymerase activity by inhibiting gene expression in DNA synthesis-related proteins such cyclin A2. These findings show that 6-MSITC suppresses FBS-stimulated cell cycle progression from G0 to S phase in quiescent JB6 cells, as well as suppressing cyclin A2 gene expression.
Author(S) Details
Takashi Hashimoto
Division of Applied Chemistry in Bioscience, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan.
Maki Kobayashi
Division of Applied Chemistry in Bioscience, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan and Faculty of Nutrition, Kobe Gakuin University, 518 Arise, Ikawadani, Nishi, Kobe, Hyogo 651-2180, Japan.
Kazuki Kanazawa
Division of Applied Chemistry in Bioscience, Graduate School of Agricultural Science, Kobe University, Rokkodai-cho 1-1, Nada-ku, Kobe 657-8501, Japan.
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