Plant biotechnology is being used as a tool for preserving natural habitats and ensuring their long-term use for ecological balance, as well as developing technologies for growing plants in a variety of cropping systems and on difficult soil, improving state-of-the-art technologies for extracting, characterization, and utilising necessary bioactive components, and generating scientific and clinical data to support the health benefits of botanical medicines. By creating micropropagation techniques, plant tissue culture can be used to rapidly reproduce virus-free planting material in plants suitable for degraded lands and temperate locations. Micropropogation is a time and space-saving approach for producing disease-free and elite viral propagules. In vitro gene bank technology allows for the storage and conservation of germplasm, allowing for the preservation of vegetatively propagated plants in cryogene banks and the storage of recalcitrant seeds, embryos, and pollens in liquid nitrogen for lengthy periods of time. This chapter covers micropropagation protocols established in our lab for a variety of plants appropriate for cultivation in undulated areas and temperate climates, as well as the detection and manufacture of natural substances found in plants and ex situ conservation techniques. Biotechnology and high-value plant biodiversity can be combined as a developmental challenge and an economic opportunity in the future.
Author(S) Details
Manoj Kumar Tripathi
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Sushma Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Niraj Tripathi
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Gyanendra Tiwari
Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Deepa Bhatt
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Megha Vibhute
Krishi Vigyan Kendra, Burhanpur, India.
Neha Gupta
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Nishi Mishra
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Prerana Parihar
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Purnima Singh
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Akash Sharma
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Ashok Ahuja
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Sharad Tiwari
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
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