In vitro differentiation of canine bone marrow mesenchymal stem cells (BMSCs) into pancreas islet-like cells is the purpose of this study. The BMSCs of healthy canines were isolated and cultured in the lab. After the third passage, BMSCs were induced to grow into islet-like cells using two-step induction techniques (at 80 percent confluence). Cell morphology, reverse transcriptase PCR (RT-PCR) and quantitative PCR (qPCR) expression of the canine insulin gene, immunocytochemistry detection of nestin and insulin proteins, and quantification of insulin level in culture media were all used to characterise differentiated BMSC cells into islet-like cells. Grape-like cell clusters might be spotted using a bright field microscope. In differentiated cells, the expression of the insulin gene and protein was identified. Insulin levels in the culture media of differentiated cells were excessively high. It was discovered that canine BMSCs can be differentiated into islet-like cells. These differentiated cells may have played an important role in the treatment of canine diabetes. Dogs, BMSCs, Islet-like cells, Differentiation are some of the key words in this study.
Author (S) Details
P. M. Deepa
Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India and Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad - 673 576, Kerala, India.
Umesh Dimri
Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.
Vikas Chandra
Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.
G. Taru Sharma
Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.
View Book :- https://stm.bookpi.org/NFMMR-V10/article/view/3501
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