Investigating the Differentiation of Canine Bone Marrow Mesenchymal Stem Cells in to Islet–Like Cells | Chapter 2 | New Frontiers in Medicine and Medical Research Vol. 10

 In vitro differentiation of canine bone marrow mesenchymal stem cells (BMSCs) into pancreas islet-like cells is the purpose of this study. The BMSCs of healthy canines were isolated and cultured in the lab. After the third passage, BMSCs were induced to grow into islet-like cells using two-step induction techniques (at 80 percent confluence). Cell morphology, reverse transcriptase PCR (RT-PCR) and quantitative PCR (qPCR) expression of the canine insulin gene, immunocytochemistry detection of nestin and insulin proteins, and quantification of insulin level in culture media were all used to characterise differentiated BMSC cells into islet-like cells. Grape-like cell clusters might be spotted using a bright field microscope. In differentiated cells, the expression of the insulin gene and protein was identified. Insulin levels in the culture media of differentiated cells were excessively high. It was discovered that canine BMSCs can be differentiated into islet-like cells. These differentiated cells may have played an important role in the treatment of canine diabetes. Dogs, BMSCs, Islet-like cells, Differentiation are some of the key words in this study.

Author (S) Details

P. M. Deepa
Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India and Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad - 673 576, Kerala, India.

Umesh Dimri
Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.

Vikas Chandra
Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.

G. Taru Sharma
Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly – 243122, Uttar Pradesh India.

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AgNOR Expression in Canine Visceral Leishmaniosis | Chapter 10 | Recent Research Advances in Biology Vol. 7

 The AgNOR technique is one method of assessing cellular metabolism. Since NORs are involved in protein synthesis, their number rises in tandem with the rise in cell metabolism that occurs when the cell begins the cell cycle. NORs are involved in active transcription during Leishmania spp infection, and depending on clinical status, their activation may result in histopathological lesions. As a result, an AgNOR marker that can distinguish Leishmania-infected dogs with a higher risk of body impairment must be found. The aim of this research was to reevaluate the relationships between histological lesions, AgNORs expression, and clinical manifestations.

Author (s) Details

Bárbara Laurice Araújo Verçosa
Institute of Biological Sciences. Federal University of Minas Gerais. Brazil.

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Sarcoptic Mange Mites in Dogs and Treatment | Chapter 4 | Current Research in Agriculture and Veterinary Sciences Vol.1

 

The aim of this study was to evaluate the efficacy of the ivermectin tablet administered orally for
Sarcoptes scabiei treatment in naturally infested dogs. Fourteen dogs (7 males and 7 females)
mongrel dogs, one to five years-old presenting positive skin scrapings for S. scabiei mites, distributed
into two groups with equal proportions of both sexes, containing seven animals per group were used
in this study. All animals were treated every 7 days, totalizing four treatments in each dog (days 0, +7,
+14 and +21). The Group I received ivermectin tablet at a dose of 0.2 mg/kg and the positive control
group (Group II) received a commercial product based on ivermectin tablet at the same dose of Group
I. Skin scrapings, clinical and laboratorial parameters analysis were performed during the
experimental period. Clinically, there were no significant differences between the two groups
evaluated prior and after treatments. Negative skin scrapings were observed in both groups from day
D+14, remaining negative until the end of the experimental period. The initial skin lesions observed in
clinical evaluation regressed from day D+14, and clinical improvement was evident in both groups.
The ivermectin tablet (Ivermectan Pet®, UCBVET Saúde Animal) administered orally was effective to
treat S. scabiei infestation in naturally infested dogs.

Author (s) Details

Gisele Maria de Andrade
Department of Research & Development (Pharmacotechnics, Physical-Chemical and Technical), UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

Moacir Marchiori Filho
Department of Research & Development (Pharmacotechnics, Physical-Chemical and Technical), UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

Marcelo Brunini
UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

Armando Leonelo Neto
Department of Research & Development (Pharmacotechnics, Physical-Chemical and Technical), UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

Raquel A. Ré
Department of Research & Development (Pharmacotechnics, Physical-Chemical and Technical), UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

Alice T. S. de Matos
Department of Research & Development (Pharmacotechnics, Physical-Chemical and Technical), UCBVET Saúde Animal. Praça Dr. Joaquim Batista, 150, Centro, Jaboticabal, SP 14870-090, Brazil.

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