Plants respond to grazing by producing chemical defense compounds such as protease inhibitors and secondary metabolites such as flavonoids, making them less palatable for feeding and negatively affecting the physiology of insects. The purpose of this study was to assess the phytochemical response of soybean cultivars Glycine max resistant (IAC-17, IAC-24) and susceptible (IAC-P1) to insects after mechanical damage. These cultivars were mechanically injured, and after 24 HPLC was used to identify and quantify flavonoids in plant samples for several hours. The flavonoids daidzein, quercetin, and rutin were measured, with daidzin having the highest concentration. soybean cultivars after mechanical damage. Rutin was biosynthesized by IAC-24. The cultivars IACPL1, IAC-17, and IAC-24 did not show a flavonoid response to mechanical damage. Flavonoids are not produced by mechanical damage in soybean cultivars.
Author(s) DetailsNeilier Rodrigues da Silva Júnior
Department of Biochemistry and Molecular Biology, Laboratory of Enzymology, Protein and Peptide Biochemistry, BIOAGRO/INCT-IPP, Universidade Federal de Viçosa, Viçosa, Brazil.
Rafael Almeida Barros
Department of Biochemistry and Molecular Biology, Laboratory of Enzymology, Protein and Peptide Biochemistry, BIOAGRO/INCT-IPP, Universidade Federal de Viçosa, Viçosa, Brazil.
Yaremis Beatriz Meriño-Cabrera
Department of Biochemistry and Molecular Biology, Laboratory of Enzymology, Protein and Peptide Biochemistry, BIOAGRO/INCT-IPP, Universidade Federal de Viçosa, Viçosa, Brazil.
Maria Goreti de Almeida Oliveira
Department of Biochemistry and Molecular Biology, Laboratory of Enzymology, Protein and Peptide Biochemistry, BIOAGRO/INCT-IPP, Universidade Federal de Viçosa, Viçosa, Brazil.
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