Development and Validation of a New RP-HPLC Method for Simultaneous Analysis of Cyclosporine A and α-Linolenic Acid in Pharmaceuticals | Chapter 3 | Novel Aspects on Chemistry and Biochemistry Vol. 8

 To test the cooperative therapeutic impact of the artificial combination of cyclosporine A and α-linolenic acid (ALA), an appropriate isocratic reverse-time HPLC technique was created. Cyclosporine A is secondhand as an immunosuppressant drug in post-organ transplantation for fear that rejection. It has existed used widely for the situation of certain autoimmune diseases to a degree severe rheumatoid, arthritis, scratching, and inflammatory skin environments. The reversed stage HPLC chromatographic method was developed to get two separate peaks for both drugs inside a short retention ending (30 min run opportunity), without some interference from excipient peaks. The chromatographic separation was realized on the C18 column (5 µm, 4.6 × 250 mm) at 50 ± 0.3 ºC. The analytes were eluted at a flow rate of 0.8 mL/brief time period using an ultraviolet indicator at 210 nm with a travelling phase of 1% v/v orthophosphoric acid in water: acetonitrile:2-propanol (25:60:25 v/v/v). The method was ratified for specificity, accuracy, linearity, veracity, and system rightness. The average retention time for cyclosporine and ALA was raise to be 16.1 notes and 18.6 minutes individually. The accuracy of the assay was inside ± 2% of the true advantage and the method was found expected linear from 1.5 μg/ml to 4.5 μg/ml for cyclosporine and 5.0 µg/ml to 15 μg/ml for ALA. The order provided discrimination based on determination among peaks and the recovery was 98% to 102%. The new RP-HPLC procedure successfully divided two peaks from the interfering chromatographic peaks of the excipients. The extreme resolution got at a comparatively lower temperature (50°C) create this method more hopeful and useful. All confirmation parameters were inside the acceptable range.

Author(s) Details:

Suvarna P. Phadatare,
Bharati Vidyapeeth’s College of Pharmacy, Sector 3A, CBD Belapur, Navi Mumbai, Maharashtra 400614, India.

Munira Momin,
SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, Mumbai University, Vile Parle, Mumbai-400056, India.

Pramod Pimplikar,
Shalina Laboratories Pvt. Ltd., Pawane, Navi, Mumbai-400705, India.

Rajesh Sirwani,
Shalina Laboratories Pvt. Ltd., Pawane, Navi, Mumbai-400705, India.

Please see the link here: https://stm.bookpi.org/NACB-V8/article/view/12311