Chemical Composition and Biological Activities of Tunisian Wild Rosemary (Rosmarinus officinalis L.) Essential Oils | Chapter 2 | Novel Aspects on Chemistry and Biochemistry Vol. 6

This study was ventured with the aim to distinguish the phytochemical composition and to evaluate the antioxidant, the uncontaminated and the anti-biofilm ventures of Tunisian wild rosemary essential oils (REOs). The synthetic composition of REOs was resolved by GC-MS and its antioxidant activity was driven using 2, 2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging arrangement. The antibacterial project, evaluated by plate diffusion and micro-something for dunking method, and the antagonistic-biofilm activity, evaluated using a crystal lilac test, were tested against two Gram-beneficial bacteria (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative strains (Escherichia coli and Salmonella typhimurium). Thirty-individual compounds were identified in REOs that represent 95.64-97.43% of the oil. The main parts of the REOs that define the chemotype were eucalyptol (40.77-55.47%), camphor (5.42-18.65%) and α-pinene (9.88- 13.83%). REOs revealed antiradical activity by restricting DPPH radical with IC50 principles ranging from 397.51 to 792.53μL/mL. The inhibition district diameter principles were determined and the results accompanied that all the tested REOs showed a forceful activity against all bacterial strains. The minimum inhibitory aggregation (MIC) varied 'tween <0.97 and 1.95 except MIC of S. typhimurium that reached 3.9μL/mL and the minimum uncontaminated concentration (MBC) ranged from 0.97 to 15.62 μL/mL. Furthermore, the intentional REOs showed potential antagonistic-biofilm activities. The percentages of restriction varied from >50% to as well 90% except for KS and KMS against Salmonella typhimurium, where the restriction rates did not beat the 37%. Similarly, in the eradication venture, the majority of the tested REOs were capable to eradicate the bacterial preinstalled biofilms accompanying rates attending the 92%. Rosemary EOs shown interesting organic activities that explain the established uses of bioactive molecules concerning this plant that could be further investigated for food and drug industries as a potential source of normal antioxidant and antibacterial bioactive compounds.

Author(s) Details:

Kheiria Hcini,
Biodiversity, Biotechnology and Climate Change Laboratory (LR11ES09), Department of Life Sciences, Faculty of Science of Tunis, University of Tunis El Manar, 2092, Tunisia and University of Gafsa, Faculty of Sciences of Gafsa, Department of Life Sciences, University Campus, Sidi Ahmed Zarroug, 2112 Gafsa, Tunisia.

Abir Bahi,
Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emarates University, P.O. Box No 15551, Al-Ain, United Arab Emirates, and Laboratory of Bioressources: Integrative Biology and Valorization (LR14 ES06), Superior Institute of Biotechnology of Monatsir, Tunisia.

Mounir Abidi,
Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LR21ES26), University of Gafsa, Faculty of Sciences of Gafsa, Department of Life Sciences, University Campus, Sidi Ahmed Zarroug, 2112 Gafsa, Tunisia.

Monia Bendhifi Zarroug,
Biodiversity, Biotechnology and Climate Change Laboratory (LR11ES09), Department of Life Sciences, Faculty of Science of Tunis, University of Tunis El Manar, 2092, Tunisia and University of Gafsa, Faculty of Sciences of Gafsa, Department of Life Sciences, University Campus, Sidi Ahmed Zarroug, 2112 Gafsa, Tunisia.

Samiha Kahlaoui,
Biodiversity, Biotechnology and Climate Change Laboratory (LR11ES09), Department of Life Sciences, Faculty of Science of Tunis, University of Tunis El Manar, 2092, Tunisia.

Maria Quílez,
Departamento de Desarrollo Rural, Enología y Agricultura Sostenible, Instituto Murciano de Investigación y DesarrolloAgrario y Medioambiental (IMIDA), La Alberca (Murcia), 30150, Spain.

Maria José Jordan,
Departamento de Desarrollo Rural, Enología y Agricultura Sostenible, Instituto Murciano de Investigación y DesarrolloAgrario y Medioambiental (IMIDA), La Alberca (Murcia), 30150, Spain.

Sondes Stambouli-Essassi,
Biodiversity, Biotechnology and Climate Change Laboratory (LR11ES09), Department of Life Sciences, Faculty of Science of Tunis, University of Tunis El Manar, 2092, Tunisia.

Please see the link here: https://stm.bookpi.org/NACB-V6/article/view/11714