A Simple Electrochemical System to Evaluate Interactions between Bauhinia monandra Leaf Lectin (BmoLL) and Cratylia mollis Seed Lectin (Cramoll) Immobilized in Nafion Nanopores with Pseudomonas aeruginosa Endophytic Strain | Chapter 1 | Recent Research Advances in Biology Vol. 9

 The interaction of lectins immobilised in Nafion nanopores with Pseudomonas aeruginosa strains was studied using an electrochemical device. BmoLL, a leaf lectin specific for galactose obtained from Bauhinia monandra leaves, interacted with P. aeruginosa strain UFPEDA598, an endophytic bacterium isolated from B. monandra leaves. Cramoll is a seed lectin from Cratylia mollis that is selective for glucose-binding proteins. In the presence of galactose, a lectin inhibitor, the effect of this interaction was an increase in electrochemical potential and stability, with a binding preference for endophytic membrane carbohydrates. Cramoll had no interaction with the endophytic strain since the electrochemical potential did not differ from the control; nonetheless, UFPEDA-39 (IT2633); after the contact, a reduction in electrical potential was detected, with stability to the glucose-inhibited lectin. In order to recognise microorganisms in real time, we devised a simple, label-free approach. The biosensor was able to detect P. aeruginosa glycoconjugate surfaces from a variety of settings, making it helpful for selective identification.

Author (S) Details

Sandra R. Souza
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Benny F. Oliveira
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Jadilma C. Pimentel
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Sergio A. F. Ramos
Departamento de Educação, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, s/n – Dois Irmãos, Recife, PE, CEP 52171-900, Brazil.

Janete M. Araújo
Departamento de Antibióticos, CB, UFPE. Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Thiago H. Napoleão
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Patrícia Maria G. Paiva
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Maria T. S. Correia
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Luana Cassandra B. B. Coelho
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, UFPE, Avenida Prof. Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

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