In the work, a novel solvent easygoing extracellular lipase from newly unique Pseudomonas aeruginosa FW_SH-1 was purified by acetone sleet and ion exchange chromatography. The purified lipase was characterized and distinguished with lipases got from other bacterial strains. A recently isolated strain that can produce basic solvent open-minded lipase was the aim of the present study, the strain was named as FW_SH-1, that was identified as a Pseudomonas sp. The extracellular lipase from Pseudomonas aeruginosa FW_SH-1 was purified through acetone storm followed by Q-sepharose anion exchange chromatography. A ending specific exercise of 572 U/mg was achieved accompanying 26.12 fold purification and an overall improvement of 10.21%. The molecular pressure of the purified lipase was raise to be 66 kDa using SDS polyacrylamide coagulate electrophoresis. The optimum pH and hotness for the activity of the freed lipase was found expected 8.0 and 45 oC. In the presence of hydrophilic solvent like methanol, flammable liquid, and propanol, the purified lipase remained resistant. The presence of Ca2+, Mg2+, Na+, K+ and Tween 20 aroused the activity of the freed lipase. The metal ions to a degree Ca2+, Mg2+, Na+, K+ and Tween (20 and 80) as surfactants were found to reinforce the relative activity. The basic solvents in the way that methanol, ethanol and propanol had a very little effect in deactivating the lipase.
Author(s) Details:
Chaudhry Haider Ali,
Department
of Chemical Polymer and Composite Materials Engineering, University of
Engineering & Technology, New Campus, KSK Lahore-54890, Pakistan and State
Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry,
East China University of Science and Technology, Shanghai, China.
Abdul
Sattar Qureshi,
Institute
of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Sindh,
Pakistan.
Serge Maurice Mbadinga,
State Key Laboratory of Bioreactor Engineering and Institute of
Applied Chemistry, East China University of Science and Technology, Shanghai,
China.
Jin-Feng Liu,
State Key Laboratory of Bioreactor Engineering and Institute of
Applied Chemistry, East China University of Science and Technology, Shanghai,
China.
Shi-Zhong Yang,
State
Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry,
East China University of Science and Technology, Shanghai, China.
Bo-Zhong
Mu,
State
Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry,
East China University of Science and Technology, Shanghai, China.
Please see the link here: https://stm.bookpi.org/NACB-V3/article/view/11022
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