The aim of work contained introduction of the composite form of hyacinth (Hyacinthus) introduced and spread in Georgia into In-vitro civilization and research of peculiarities of morphogenesis thereof. Various cultivars of hyacinths have the flowers of miscellaneous colour, size and shape. They are cultured on well-lighted, abundant loamy soil. It is cultured by bulbs, rarely by children. Oil produced from hyacinth flowers is used in perfumery. We doubled the sterilising agent and allure exposure accompanying the intention of acquiring the sterile breedings. In addition, we started accompanying a hyacinth bulb peel explant to conceive the embryogenic callus. Aiming initiation of callusogenesis and embryogenesis, we tested and studied concurrent fair to benzylaminopurine and auxins (NAA and IBA) in concentrations correlation of 2:10; 2:15; 2:20. We established the conditions for the embryoid's development and the regeneration of the bodily embryos after evolving the embryogenic calli and the somatic embryoids. We caused aseptic cultures of hyacinth; grown the hormone content of the food medium for induction of embryogenic accustom cultures; Callus tissues were very embryogenic and preserved this feature for two and more age period; Embryoids introduced and maturated on the callus fabric acynchronically; we developed the mineral mediums enough for maturating and regeneration of the embryoids. The created embryocultures well reproducted and rooted providing origin of mictrobuds on the rootlets.
Author(s) Details:
Nana Zarnadze,
Cell
Technology Laboratory, Faculty of Natural Sciences and Health, Batumi Shota
Rustaveli State University, Georgia.
Ketevan
Dolidze,
Department
Botany, Faculty of Natural Sciences and Health Care, Batumi Shota Rustaveli
State University, Georgia.
Sophiko Manjgaladze,
Cell Technology Laboratory, Faculty of Natural Sciences and Health,
Batumi Shota Rustaveli State University, Georgia.
Tsiala Bolkvadze,
Cell Technology Laboratory, Faculty of Natural Sciences and Health,
Batumi Shota Rustaveli State University, Georgia.
Inga Diasamidze,
Department
Botany, Faculty of Natural Sciences and Health Care, Batumi Shota Rustaveli
State University, Georgia.
Please see the link here: https://stm.bookpi.org/CERB-V8/article/view/10927
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