Purification and Characterization of Organic Solvent Tolerant Lipase from Newly Isolated Pseudomonas aeruginosa FW_SH-1 | Chapter 3 | Novel Aspects on Chemistry and Biochemistry Vol. 3

 In the work, a novel solvent easygoing extracellular lipase from newly unique Pseudomonas aeruginosa FW_SH-1 was purified by acetone sleet and ion exchange chromatography. The purified lipase was characterized and distinguished with lipases got from other bacterial strains.  A recently isolated strain that can produce basic solvent open-minded lipase was the aim of the present study, the strain was named as FW_SH-1, that was identified as a Pseudomonas sp. The extracellular lipase from Pseudomonas aeruginosa FW_SH-1 was purified through acetone storm followed by Q-sepharose anion exchange chromatography. A ending specific exercise of 572 U/mg was achieved accompanying 26.12 fold purification and an overall improvement of 10.21%. The molecular pressure of the purified lipase was raise to be 66 kDa using SDS polyacrylamide coagulate electrophoresis. The optimum pH and hotness for the activity of the freed lipase was found expected 8.0 and 45 oC. In the presence of hydrophilic solvent like methanol, flammable liquid, and propanol, the purified lipase remained resistant. The presence of Ca2+, Mg2+, Na+, K+ and Tween 20 aroused the activity of the freed lipase.  The metal ions to a degree Ca2+, Mg2+, Na+, K+ and Tween (20 and 80) as surfactants were found to reinforce the relative activity. The basic solvents in the way that methanol, ethanol and propanol had a very little effect in deactivating the lipase.

Author(s) Details:

Chaudhry Haider Ali,
Department of Chemical Polymer and Composite Materials Engineering, University of Engineering & Technology, New Campus, KSK Lahore-54890, Pakistan and State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.

Abdul Sattar Qureshi,
Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Sindh, Pakistan.

Serge Maurice Mbadinga,
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.

Jin-Feng Liu,
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.

Shi-Zhong Yang,
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.

Bo-Zhong Mu,
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China.

Please see the link here: https://stm.bookpi.org/NACB-V3/article/view/11022

Degradation of Polyester Partially Oriented Yarns through Alkaline Hydrolysis Process: A Recent Study | Chapter 05 | New Approaches in Engineering Research Vol. 12

 The feed material for the draw texturing process of polyester regular and micro fibres is partially oriented yarn. The biggest challenge the industry faces is POY quality, because storage of POY causes it to degrade, and the quality of draw textured yarn will be questioned as a result. As a result, a method to verify the freshness of POY is required, and alkaline hydrolysis can be employed as a tool to investigate the structure of POY. In this study, an earnest attempt is made to investigate the caustic impact on polyester POY using various degrading process parameters. This is the first study to use alkaline hydrolysis as a technique to better understand the deterioration process. The experimental methodology consisted of selecting different pet poy (both micro and macro denier) and storing them in standard conditions according to standard procedures for degradation, and then subjecting the stored materials to alkaline hydrolysis according to Zeronian and Collin's standard procedure. The density, critical dissolving time, and molecular weight of the treated samples were measured. The deterioration process has a major impact on these qualities in all circumstances, as evidenced by the weight loss of the alkaline hydrolysis process. In addition, the results were statistically significant.

Author(S) Details

J. Hayavadana
Department of Textile Technology, University College of Technology, Osmania University, Hdyerabad-7, India.

View Book:- https://stm.bookpi.org/NAER-V12/article/view/3839

Degradation of Polyester Partially Oriented Yarns through Alkaline Hydrolysis Process: A Recent Study | Chapter 5 | New Approaches in Engineering Research Vol. 12

The behaviour of strut-and-tie models (STMs) as derived from truss analogue models is the subject of this chapter. STMs are frequently used to assess and model the internal force distribution of a reinforced concrete deep beam structure from the point load to the supports that carry bending, shearing, and twisting forces in disturbed zones (D-regions). Where the stress situation occurs idealised as the concrete strut, the steel tie, and the nodal area, the action of the strut and tie creates an increase in the strength of the high-reinforced concrete beam construction, the D-region on structural elements can be more easily studied. The deep beam was numerically modelled using compressive loading simulation until it collapsed. To determine deflection, cracking, and destruction of ultimate load, model modification of two diagonal reinforcements, diagonal symmetrical truss reinforcement, and diagonal frame truss reinforcement, ANSYS 3D half-span symmetrical Strut-and-Tie model deep beams modelling was done. The ultimate bending capacity, load-deformation, ductility, stress behaviour, strain, and fracture pattern of STMs have all been determined through shape modification. The numerical modelling result reveals a significant difference in STM's deep beam behaviour.

Author (S) Details

J. Hayavadana
Department of Textile Technology, University College of Technology, Osmania University, Hdyerabad-7, India.

View Book :- https://stm.bookpi.org/NAER-V12/article/view/3839