The genomic DNA of probiotic isolates from the stomach, as well as the gene sequence responsible for enzyme activity in Labeo rohita, were examined in this work. Probiotics are helpful microbial communities that change probiotic bacterial strain gut colonisation to boost fish development and immunity in difficult environmental situations. The experiments were conducted by combining the strain with water and feeding it with commercial feed for the growth of Labeo rohita. According to the findings, only two strains demonstrated higher activity. Using Universal 16S primers, the genomic DNA of these isolated probiotic microbes was analysed and electrophoresed to determine their taxonomic identification. The 16S rRNA sequences of the isolates were amplified using primers and PCR. The genomic DNA of the isolated probiotic bacteria was examined with Universa 16S primers and electrophoresed to validate the taxonomic identification. The isolates' 16S rRNA sequences were amplified using primers and PCR. Blast analysis of the 16S rRNA sequence was performed using sequences from the NCBI database. Amylase, protease, and lipase enzymes improve water quality indicators, food absorption, and L. rohita proliferation, all of which promote probiotic effectiveness. The impact of the discovered strain on fish development was explored, and it was recommended that it may be utilised to boost the growth of L. rohita fish.
Author(s) Details:
M. Abareethan,
Department of Zoology, Government Arts College (Autonomous), Salem, 636007, Tamil Nadu, India.
A. Amsath,
Department of Zoology, Khadir Mohideen College, Adirampattinam, 614701, Tamil Nadu, India.
Please see the link here: https://stm.bookpi.org/RABS-V3/article/view/7044
No comments:
Post a Comment