Histopathological lesions have long been used as biomarkers to measure the health of organisms exposed to contaminants, and they can also be used as early warning indications of illness. Because there are limited data on histomorphological alterations in the kidney after exposure to the pesticide chlorpyrifos, we decided to perform this study.
Methods: 45 inbred adult Wistar albino rats, both sexes, weighing 145–165 gms, were used in this study. At random, these animals were divided into three groups: A, B, and C. Oral chlorpyrifos was given to groups B and C at doses of 5 mg/kg body weight and 10 mg/kg body weight, respectively. The control group, Group A, was designated as such and remained so. Three mice from each group were sacrificed after 1 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks of the experiment to see the histological changes in the kidney architecture.
In Group A, there are no histological alterations. There were no histological alterations in the kidneys of Group B after one week. There was glomerulus shrinkage, tubular dilatation, glomerular hypercellularity, tubular epithelial hypertrophy, degeneration of renal tubules, and eosin positive material deposits in the glomerulus and renal tubules at the start of treatment. In the interstitium, there was lymphocyte infiltration and increased vascularity in the form of dilated arteries, fibrosis, and interstitial oedema. All of these changes pointed to glomerulonephritis, acute tubular necrosis, and interstitial nephritis, all of which can lead to acute renal failure, which can then proceed to chronic renal failure. Group C rats' kidneys revealed fewer glomeruli and renal tubular epithelial hypertrophy after 1 week of Chlorpyrifos treatment.
From the second to the eighth week, the discrepancies between Group A and Group B were increasingly obvious.
Conclusion: Chlorpyrifos generated considerable
histomorphological alterations in the kidneys of rats in the current
investigation. These modifications were distinct from those observed in control
rats. As a result, this research offered insight on chlorpyrifos' kidney
toxicity, which was found to be considerable at high dose levels.
Author(S) Details
Rekha Bhasin
JK Health Services, Jammu &
Kashmir, India.
Sajad Hamid
Sher I Kashmir Institute of Medical Sciences and Medical College, Srinagar,
Jammu & Kashmir, India.
Sunanda Raina
Govt. Medical College, Rajouri, Jammu & Kashmir, India.
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