Determination of Antimicrobial Activity of Herbal Toothpaste against Organisms Causing Dental Caries | Chapter 6 | New Visions in Biological Science Vol. 6

 Background: Dental caries is caused by bacteria producing acid, which eats away at the enamel and dentin beneath it. A study was conducted to isolate microorganisms that cause dental caries in people of various ages and determine the antimicrobial activity of various toothpastes against these germs.

The goal of this study is to isolate microorganisms that cause dental caries in people of various ages.

2. To test herbal toothpaste's antibacterial efficacy against the pathogenic organism that causes dental caries. Between June 2016 and December 2017, samples were collected at Krish Multispeciality Clinic and Prashanth Dental Clinic in Nagole and processed at the Microbiology Department of St. Francis College for Women in Hyderabad.

Methodology: Ten samples were taken from each age group, ranging from 10 to 20 years old, 20 to 40 years old, and 40 to 60 years old. The species were identified using a combination of biochemical tests and molecular characterization based on 16s rRNA type, as described in Bergey's Manual of Systematic Bacteriology. The agar well diffusion method was used to investigate the antibacterial activity of herbal toothpastes using identified organisms. For statistical analysis, ANOVA is utilised.

Because of their efficacy, Patanjali herbal medicines such as Dantkanti are getting a lot of traction and have become a part of everyday life for the common man. Patanjali toothpaste, out of all the toothpastes tested, has good antibacterial activity against all of the isolates, according to our findings. Patanjali has strong antibacterial action against all isolates examined, according to a comparative study of f-ratio and P significant value (.05.) of all pairs. Meswak's efficacy is almost identical to that of Patanjali. When compared to the other two toothpastes, Dabur red and Colgate herbal have moderate activity.

Author(S) Details

M. Shailaja Raj
Department of Microbiology, St. Francis College for Women, Hyderabad, India.

R. Hema
Department of Microbiology, St. Francis College for Women, Hyderabad, India.

Anitha Thomas
Department of Microbiology, St. Francis College for Women, Hyderabad, India.

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