Study on Evaluation of Immune Response to Enterotoxaemia Vaccine in Sheep Reared Under Experimental and Field Conditions by Using ELISA | Chapter 2 | Research Aspects in Agriculture and Veterinary Science Vol. 4

 The immunological response of vaccinated animals is measured in order to determine when booster vaccinations should be given. Serological tests can be used for seromonitoring, with ELISA being the most specific and sensitive. As a result, the goal of this study was to use ELISA to evaluate the immunological response in sheep that had been vaccinated against ET. Using an enzyme-linked immunosorbent assay, the immune response to the enterotoxaemia vaccine (ET) was assessed in sheep grown in the lab and in the field (ELISA). Six sheep were vaccinated with a booster dose on the 14th day and tested for immune response in an experimental setting; however, serum samples from 386 ET vaccinated sheep (139 from organised farms and 247 from the unorganised sector of Andhra Pradesh) were tested for protective antibody titre using ELISA in a field setting. The highest protective titre was found in sheep raised in an experimental setting on day 30 following vaccination, followed by a progressive decline until day 90. When compared to sheep kept in experimental/laboratory circumstances, immune responses of sheep kept in rural environments were shown to be low. The protective titers in sheep kept under village circumstances lasted up to 3 months, while those kept in experimental conditions lasted up to 4 months. As a result, improved management methods combined with ET booster vaccination in farms may result in a greater immune response in sheep against ET.

Author(S) Details

Nagendra Reddy Thopireddy
Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkatewara Veterinary University, Tirupati, Andhra Pradesh, India.

Sreenivasulu Daggupati
Department of Veterinary Microbiology, College of Veterinary Science, Sri Venkatewara Veterinary University, Tirupati, Andhra Pradesh, India.

Eswara Prasad Pagadala
Department of Veterinary Biochemistry, College of Veterinary Science, Sri Venkatewara Veterinary University, Tirupati, Andhra Pradesh, India.

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