Aim: The goal of this study is to establish an analytical method for determining drug concentrations or analyses in synthetic mixes including both antifungal drug Luliconazole and corticosteroid medicine Clobetasol Propionate, regardless of their composition.
The approach was validated to meet the requirements of the International Conference on Harmonization (ICH). On a reversed-phase Inertsil C18 column (5 m, 250mm x 4.6mm i.d) with a mixture of Acetonitrile: Water pH adjusted with H3PO4 (60: 40) and UV detection at 264 nm, chromatographic separation was performed using an isocratic approach. With a 20L injection volume, the compounds were eluted at a flow rate of 1.0 mL/min.
The calibration curves for Luliconazole and Clobetasol Propionate were linear (r2 > 0.999) for the concentration ranges of 10-200 g/mL and 5-100 g/mL, respectively. Luliconazole and Clobetasol Propionate had average retention durations of 3.16 and 6.94 minutes, respectively. The proposed method's percent RSD was determined to be less than 2 percent. The devised method's percent recovery was determined to be between 99.22 and 99.48 percent.
Conclusion: Because the devised approach is simple, quick, precise, and accurate, it was successfully used to determine Luliconazole and Clobetasol Propionate in a synthetic mixture.Author(S) Details
Binal Solanki
Nootan Pharmacy College, Sankalchand Patel University, Visnagar, Laxminarayan Dev College of Pharmacy, Bharuch, India.
Hirak Joshi
Faculty of Pharmacy, Nootan Pharmacy College, Sankalchand Patel University, Visnagar, India.
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