Ciprofloxacin is a fluoroquinolone of the second generation and one of the most successful and extensively used medications in the fluoroquinolone class. Uncommon ciprofloxacin side effects include bone marrow suppression, thrombocytopenia, anemia, agranulocytosis, renal failure, and others. Most research focused on lutein, a xanthophyll (an oxygenated carotenoid) that has considerable antioxidant activity in vitro and has also been linked to lowering the risk of age-related diseases. The goal of the study was to explain the function of apoptosis by measuring the Bcl-2 associated X protein (Bax) marker, as well as the processes of ciprofloxacin-induced bone marrow toxicity, and to see if lutein might protect rats' bone marrow from ciprofloxacin-induced toxicity. Thirty-six Sprague-Dawley rats were divided into six groups (each with six animals) at random: Rats in Group I (control) received a single oral daily dose of liquid paraffin (4ml/kg body weight/day) by oral gavage for 25 days; rats in Group II (ciprofloxacin-treated) received a single oral daily dose of liquid paraffin (4ml/kg body weight/day) for 25 days and then 500 mg/kg ciprofloxacin by oral gavage. Groups V and VI were given an oral dose of lutein (6 mg and 24 mg/kg/day), respectively, by oral gavage for 25 days (lutein-treated); and Groups V and VI were given an oral dose of lutein (6 mg and 24 mg/kg/day), respectively, by oral gavage for 25 days (lutein+ ciprofloxacin). When compared to control (Group I) rats, ciprofloxacin (Group II) induced significant (P0.05) reductions in total RBCs and -WBCs, as well as significant (P0.05) elevations in Bcl-2 associated X protein (Bax) in bone marrow (BM) tissues homogenates. Orally administered lutein to rats (Groups and V) resulted in non-significant changes (P>0.05) in total -RBCs and -WBCs, as well as non-significant differences (P>0.05) in total -RBCs and -WBCs. Bax levels in BM tissues homogenates were non-significant (P>0.05) when compared to similar values in Group rats. Orally administered lutein with ciprofloxacin (Groups V and VI) caused a significant increase (P0.05) in total RBCs and WBCs, as well as a significant decrease (P0.05) in Bcl-2 associated X protein (Bax) in bone marrow (BM) tissues homogenates, when compared to the corresponding levels in the ciprofloxacin-treated group of rats (Group II). The current study's findings suggested that lutein could be a beneficial chemical for reducing ciprofloxacin-induced bone marrow damage.
Author(s) Details
Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.Nwamkwere Godson
Nada N. Al-Shawi
Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
View Book :- https://stm.bookpi.org/CAPRD-V1/article/view/3317
No comments:
Post a Comment