In the present study, paper biosensor was
developed using Tyr-AuNps bioconjugate produced by
Streptomyces for the detection of phenol
from the effluent of wine, paper and plastic industries.
Among three filter papers assessed, Whatman No.2 filter paper was proved to be
the best paper
base for the development of biosensor. Tyrosinase and gold nanoparticles being
produced by a
single novel isolate Streptomyces tuirus DBZ39, proved to be efficient bioconjugate for the detection
of phenol constituents, due to its biocompatibility. The substrate specific
catalytic activity of the
tyrosinase and unique Surface Plasmon Resonance attribute of gold nanoparticles
are the cause for
efficient detection of phenol constituents from the effluent of wine, paper and
plastic industries in 3
min. The different types and quantity of phenolic constituents in various
industrial effluents, such as
phenol in wine, dopamine in paper and catechol in plastic effluents were
accurately detected by the
bioconjugate. The efficacy of tyrosinase in the detection of phenol
constituents was expected to be
enhanced by the gold nanoparticles because of their electron, optical and
magnetic properties. This
novel paper strip biosensor could be cost effective and efficient means of
future devices for the
detection of phenolic pollutants from any environmental samples.
Author(s) Details
Dr. Bi Bi Zainab Mazahri
Department of Microbiology, A-DBT Research Laboratory, Gulbarga
University, Kalaburagi 585 106, Karnataka, India.
Prof. Dayanand Agsar
Department of Microbiology, A-DBT Research Laboratory, Gulbarga
University, Kalaburagi 585 106, Karnataka, India.
Dr. M. V. N. Ambika Prasad
Department of Microbiology, A-DBT Research Laboratory, Gulbarga
University, Kalaburagi 585 106, Karnataka, India.
View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/235
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