The production of polyhydroxyalkanoates (PHAs) using raw
sewage has been previously attempted to derive value from waste; however, the
yields of PHAs were low. Therefore, we attempted to increase the yield by
applying different strategies, such as supplementation of sewage with
additional carbon sources, dilution of high-carbon-containing wastewater
(cheese whey) with low-carbon-containing wastewater (sewage), and concentration
of sewage to improve the available carbon source. After applying these
strategies, the PHA production capacity of the bacterium Bacillus sp. CYR1 is
enhanced 22-fold. CYR1 incubated using cheese whey (CW) diluted with sewage
showed higher PHA production than those incubated using CW diluted with
distilled water. The PHA produced in this study was identified as poly-𝛽-polyhydroxybutyrate
(PHB), and the characteristics of the produced PHB were comparable to those of
standard PHB. We believe that our study makes a significant contribution to the
literature because we show the potential of CYR1 strain in PHB production using
low- or high-carbon-containing wastewater. The reuse of sewage will help to
reduce the ecological burden and energy consumption on a global scale.
The increasing burden placed by plastic waste on the
environment has necessitated the protection of our planet from environmental
disasters. Thus, switching from limited natural and fossil resources to PHAs
for plastic production matches today's concrete initiatives to reach the global
Climate and Sustainable Development Goals. On a related note, the constant generation
of sewage in our daily activities accounts for a high proportion of the
environmental load, and the energy and cost required for sewage treatment are
extensive. Thus, the reuse of sewage is essential for reducing the ecological
burden and energy consumption on a global scale. Consequently, painstaking
efforts have been taken to transform waste management into resource management.
Our group has been working on the production of bioplastics using rejected
materials. Therefore, in this study, we aimed to enhance PHA production from
sewage by applying different strategies using Bacillus sp. CYR1. A high rate of
glucose consumption (83.6%) was also observed. Higher phaC gene expression
levels were observed in high-carbon-containing sewage but at lower nitrogen
concentrations. Based on these analyses, it was confirmed that the CYR1 strain
can be useful for producing PHA using low- or high-carbon-containing
wastewater.
Author(s) Details
Young-Cheol Chang
Course of Chemical and Biological Engineering, Division of
Sustainable and Environmental Engineering, Muroran Institute of Technology,
27-1 Mizumoto, Muroran, Hokkaido 050-8585, Japan..
Please see the book here:- https://doi.org/10.9734/bpi/rdcbr/v3/7908C
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