Background: Chick embryo is one of the most commonly used animals
to study the adverse effects of various drugs for research purposes. For
ethical reasons, animal models are frequently used in research. Currently, type
2 diabetes mellitus is treated by using the medication lixisenatide.
Therapeutically, it is thought to be superior to other GLP1 receptor
antagonists for the treatment of type 2 diabetes.
Objectives: The primary objective of this study is to understand
the adverse effects of lixisenatide on the kidneys of developing chick embryos.
Materials and Methods: This study was conducted at the Department
of Anatomy, Santosh Medical College, Ghaziabad, Uttar Pradesh, India. The
starting human dose of lixisenatide was 10 mcg subcutaneously daily for 14 days
and increased further to 20 mcg daily lifelong. The chick embryos were
dissected after being sacrificed, and the kidneys from both sides were
separated and kept in a 10% formaldehyde solution. Under flowing water, the
tissues were cleaned. To learn about the typical histological characteristics
of the kidneys, the sections were examined by using a light and compound
microscope. The obtained data were subjected to statistical analysis.
Results: Minute vacuolation with or without fat globules in the cytoplasm
of the lining epithelium of proximal and distal convoluted tubules was observed
in experimental groups C, D, and E. The lymphocytic infiltration was higher in
cortical areas of kidneys. As the dose of Lixisenatide increases with each
experimental group, the histopathological changes in the kidney also show more
changes with each experimental group, and these are statistically significant
(p<0.05).
Conclusion: The medicine has some negative effects on the kidneys
of the chick embryo, as evidenced by the observations (vacuolation in DCT,
congestion in glomeruli, lymphocytic infiltration, etc.). Therefore, if it tend
to use this vital medication carelessly patient can develop renal issues and
resistance. A limitation of this study is that it was conducted solely on a
chick embryo model. Further study must be performed in mammal research models
for a better understanding of the effects of Lixisenatide in humans.
Author
(s) Details
Amit
Kumar Srivastava
Department of Anatomy, Santosh Medical College, Gaziabad, Uttar
Pradesh, India.
Yogesh
Yadav
Department of Anatomy, Noida International Institute of Medical
Sciences, Noida, Uttar Pradesh, India.
Ashok
Kumar Gupta
Department of Anatomy, Government Medical College, Saharanpur,
Uttar Pradesh, India.
Please see the book here:- https://doi.org/10.9734/bpi/rpbs/v4/5500
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