Primary tumours of the central nervous system are a rare
group of diverse tumours that account for less than 2% of all tumours, but are
the fourth most common cause of cancer death. The role of element homeostasis
in neoplastic disease pathogenesis is beyond question. The imbalance of trace
elements precisely underlies the initiation and promotion of tumour pathology.
The aim of the study was to investigate blood and tissue macroelements,
microelements and hemoproteins levels in brain tumours and their intermolecular
interactions. Samples of blood and brain tumour tissues were taken from 12
patients with malignant brain tumours (glioma, glioblastoma, astrocytoma).
Samples of tissues were taken from 7 patients who died as a result of trauma
related to a road traffic accident, without connection to head and brain
injury. Detection of myoglobin level was implemented by the reaction of passive
hemagglutination and an immunoturbidimetric test. Catalase activity was
determined by the method of Beer and Sizer. Free radical activity was
determined by the method of induced biochemiluminescence. The microelements
level was investigated using atomic emission spectrometry. Statistical data
processing was performed using STATISTICA Base software (StatSoft Inc.). To
build the networks of studied hemoprotein interactions with signalling pathways
of proteins, expressed in brain tumours, molecular interaction databases
(STRING, BioGrid) were used. Modern databases of signalling pathways (KEGG)
suggest that in normal cells, hypoxia can lead to HIF-1A protein synthesis. ROS
synthesis inhibits the PHD enzyme and triggers the release of calcium ions, and
increases proliferation. Calcium ions are a triggering factor of apoptosis and
cell proliferation. In this study, a significant increase (more than 7 times)
of calcium concentration in blood and brain tumour tissue was detected,
compared with healthy people. Myoglobin can possibly be the cell adaptation
factor towards hypoxia, oxidative stress and element homeostasis violation, and
myoglobin level decreasing can additionally stimulate proliferation, by
apoptosis inhibition. This is one of the first studies to have examined
intermolecular relationships between microelements, hemoproteins and
antioxidant enzymes in gliomas, and future studies are required to validate
these findings and explore their potential diagnostic and therapeutic
implications.
Author(s) Details
Yazykova A.B.
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
Obukhova L.M.
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
Medyanik I.A.
Nizhny Novgorod Research Institute of Traumatology and Orthopedics of
Public Health Ministry of Russian
Federation, Nizhny Novgorod, Russia.
Yashin K.S
Nizhny Novgorod Research Institute of Traumatology and Orthopedics of
Public Health Ministry of Russian Federation, Nizhny Novgorod, Russia.
Pimenov V.G.
Institute of Chemistry of High-Purity Substances of the Russian Academy of
Sciences, Russia.
Evdokimov I.I
Institute of Chemistry of High-Purity Substances of the Russian Academy of
Sciences, Russia.
Barinova O.V.
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
Please see the book here :- https://doi.org/10.9734/bpi/msraa/v10/5124
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