Effect of Nano Selenium-enriched Diet on the Growth Performance and Biochemical Parameters of Labeo rohita | Chapter 9 | Research Perspective on Biological Science Vol. 6

 

Selenium (Se) is an essential microelement utilised in aqua-feeds for aquatic animals’ normal growth, well-being and health. When selenium is present in adequate concentrations in fish diets, protecting against oxidative damage. Selenium is required for the function of several significant seleno-proteins, including glutathione peroxidase (GPx) responses of fish. This study was intended to assess the growth performance of Labeo rohita (Rohu) resulting from the nano Se-merged diet. Nano Se was synthesized and its physico-chemical characteristics were characterized using UV-VIS, SEM, EDAX, XRD and FTIR for varying quantities of nano Se; Diets - I-0, II- 0.5, III-1, IV-1.5, V-2 and VI- 2.5 mg/ kg were prepared, making use of fish meal (FM), groundnut oil cake (GNOC), wheat flour (WF) and tapioca flour (TF). Rohu fingerlings (2 ± 0.5 g) were acclimated for 2 weeks and stocked in triplicate to evaluate growth performance and biochemical parameters under experimental feeding conditions. Feed-utilising parameters and the biochemical composition of Rohu were evaluated subsequently after 28 days. Data on growth parameters were analysed using IBM SPSS software 17. The UV-Vis Spectroscopy revealed that nano Se was assessed in wavelengths of 200 to 500 nm and exhibited strong absorption at 322 nm. The SEM image showed spherical morphology with an average particle diameter of 12.22 μm. EDAX spectrum recorded two signals at 1.5 keV and 11 keV. XRD patterns showed crystalline characteristics of nano Se at 2θ correlators of 23.5o, 29.7o, 41.4o, 43.6o, 45.4o, 51.7o, 55.9o and 61.5o. FTIR spectrum was examined in the range of 4000 – 500 cm-1. Rohu's growth performance and biochemical analysis revealed that the protein, carbohydrate and lipids of gill, liver and muscle of Rohu were highly increased in the case of diet IV containing 1.5 mg/kg nano Se. The study concluded that a fish diet containing 1.5 mg/kg of nano Se is the recommended level for Rohu's effective growth and biochemical criteria.

 

 

Author(s) Details

 

M. Dayana Senthamarai
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, TN, India.

 

M. Rekha
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, TN, India.

 

M. R. Rajan
Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, TN, India.

 

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