Determining the Effect of Low Gamma Radiation and Methyl Jasmonate on the Regeneration of Vanilla planifolia | Chapter 7 | Research Highlights in Science and Technology Vol. 8

 This phase highlight the effect of reduced dosage of gamma radiation and methyl jasmonate on the conversion of Vanilla planifolia, a tropical crawling orchid from the family Orchidaceae. Somaclonal alternatives obtained by artificial mutagenesis with gamma radiation are innocent regulatory restraints and allow the regeneration of hereditary variations in a short magnitude at low cost, increased which this system supplies easy guidance of explants in confined and controlled rooms under aseptic environments. V. planifolia plant was surface sterilized before inoculated on Murashige and Skoog medium and hatched for three weeks at 25±2ºC. After incubation, the ideas were exposed to three doses of gamma radiation (0 Gy, 10 Gy, 20 Gy and 30 Gy). Then, the breedings were directly moved into five different concentration of methyl jasmonate (0 µM, 10 µM, 20 µM, 40 µM and 80 µM). After ten weeks, the result demonstrated that regeneration of V.planifolia was chief at 20 µM methyl jasmonate media subsequently exposed to 10 Gy irradiation, place shoot altitude was 14.5±0.4 cm. Internal metabolism in plant depend well on photosynthesis because photosynthesis is the fundamental beginning of energy for growth and growth. The number of leaf, root length and root number still showed the highest for 10 Gy civilizations in 20 µM methyl jasmonate while the lowest conversion was collectively acquired from 80 µM methyl jasmonate. However, no morphological changes were detected completely treatments.  Gamma luminescence from sun or other source and methyl jasmonate have a high potential for boosting simple culture conversion. However, only low dose gamma radiation and methyl jasmonate concentration developed in a beneficial outcome, inasmuch as high application and concentration resulted in a negative consequence.

Author(s) Details:

Ellia Kartini Mujar,
Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam, Malaysia.

Norrizah Jaafar Sidik,
Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam, Malaysia.

Noor Anilizawatima Sulong,
Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam, Malaysia.

Siti Safura Jaapar,
Faculty of Pharmacy, Universiti Teknologi Mara, Puncak Alam, Malaysia.

Muhammad Hanzalah Othman,
Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam, Malaysia.

Please see the link here: https://stm.bookpi.org/RHST-V8/article/view/11543