LM, Micromorphology, SEM of Leaf and Molecular Characterization of the Genus Cinnamomum (Family Lauraceae) Found in Sri Lanka: Taxonomic Significance

 Family Lauraceae is individual of the most main plant families since appendages of this main family are used for various purposes such as bread, spices and medicines. Sri Lanka is the principal global real cinnamon (C. zeylanicum) builder (80-90%) in the world. Apart from C. zeylanicum, there are seven underutilized Cinnamomum class. For efficient exercise approaches of the Cinnamomum species and proper preservation purposes, correct identification and description of these species are very important. Environmental pliancy may considerably influence the plants' morphology making it difficult to calculate only on vegetative makeup and may not be a reliable source of news to differentiate the Cinnamomum class. Flowers are rarely found and blooming time changes among the species. Therefore, this study proposed to examine the leaf plants using standard contracts for light microscopy and scanning electron microscopy (SEM) of the eight variety of Cinnamomum that occur in Sri Lanka to judge their potential utility in taxonomic differentiation. Moreover, microscopic characterization concerning this genus using various universal barcoding domains of the cpDNA (trnL intron, the trnT-trnL, trnL-trnF, and trnH-psbA intergenic spacers) and internal transcribed spacer (ITS) of basic ribosomal DNA (rDNA) to check whether these sequences are trustworthy for species discrimination. Internal plants and leaf surface micromorphology were studied utilizing standard light microscopy and scanning electron microscopy individually. Some key petiole physical characteristics were stone container characters, the construction of vascular bundle, the presence or absence of feathered extensions, the ghost or absence of trichomes, and the shape and outline of the petiole. Leaves were hypostomatic accompanying no special composition for epidermal pavement cells. Simple, unicellular, unbranched, single and non-glandular trichomes were observed indifferent species on both surfaces of the leaves. Both adaxial and abaxial surfaces in C. dubium and C. rivulorum were laboriously covered accompanying simple, unicellular/sickle-shaped, long and thin trichomes. Transverse sections of leaves along the midrib were various in shape (well-proportioned, asymmetrical, boat, uneven, saucer). The midrib held one open arch, a central vascular bundle that was accompanying different methodical (oval, elongated, uneven, ‘V’, partially dissected into 2 or 3 slices) in different species. Micromorphology of cuticular matters on both abaxial and adaxial surfaces was various within and between class. Both abaxial and adaxial surfaces had undulating, dense cuticles with different patterns of fuller deposition. Although it was likely to identify the single nucleotide variety (SNP) of different worldwide barcoding regions of the cpDNA of different variety, no sufficient series data were obtained to segregate the species right.

Author(s) Details:

Pushpa Damayanthi Abeysinghe,
Department of Botany, Faculty of Science, University of Ruhuna, Matara, Sri Lanka.

Please see the link here: https://stm.bookpi.org/CERB-V9/article/view/11174